Ability of various alkylating agents to induce adaptive and SOS responses: A study with lacZ fusion

Masanori Otsuka, usaku Nakabeppu, Mutsuo Sekiguchi

研究成果: Contribution to journalArticle査読

31 被引用数 (Scopus)

抄録

We used alkA′-lacZ′ and umuC′-lacZ′ fused genes and determined the ability of various alkylating agents to induce adaptive and SOS responses. The degree of induction of expression of these genes was quantitatively measured by a simple colorimetric assay of ß-galactosidase activity. SN1 type methylating agents, such as N-methyl-N′-nitro-N-nitrosoguanidine and B-methyl-N-nitrosourea, were more effective inducers for the alkA than for the umuC system, while SN1 type ethylating agents, such as N-ethyl-N′-nitro-N-nitrosoguanidine and N-ethyl-N-nitrosourea, were more potent inducers for the umuC than for the alkA system. Similar but less striking effects on the two systems were obtained with SN2 type alkylating agents.

本文言語英語
ページ(範囲)149-154
ページ数6
ジャーナルMutation Research DNA Repair Reports
146
2
DOI
出版ステータス出版済み - 9 1985

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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