Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS

Kenichi Hirano, Tomohiro Ohgomori, Kazuyoshi Kobayashi, Fumiaki Tanaka, Tomohiro Matsumoto, Takamitsu Natori, Yukihiro Matsuyama, Kenji Uchimura, Kazuma Sakamoto, Hideyuki Takeuchi, Akihiro Hirakawa, Akio Suzumura, Gen Sobue, Naoki Ishiguro, Shiro Imagama, Kenji Kadomatsu

研究成果: Contribution to journalArticle査読

27 被引用数 (Scopus)

抄録

Biopolymers consist of three major classes, i.e., polynucleotides (DNA, RNA), polypeptides (proteins) and polysaccharides (sugar chains). It is widely accepted that polynucleotides and polypeptides play fundamental roles in the pathogenesis of neurodegenerative diseases. But, sugar chains have been poorly studied in this process, and their biological/clinical significance remains largely unexplored. Amyotrophic lateral sclerosis (ALS) is a motoneuron-degenerative disease, the pathogenesis of which requires both cell autonomous and non-cell autonomous processes. Here, we investigated the role of keratan sulfate (KS), a sulfated long sugar chain of proteoglycan, in ALS pathogenesis. We employed ALS model SOD1G93A mice and GlcNAc6ST-1-/- mice, which are KS-deficient in the central nervous system. Unexpectedly, SOD1G93AGlcNAc6ST-1-/- mice exhibited a significantly shorter lifespan than SOD1G93A mice and an accelerated appearance of clinical symptoms (body weight loss and decreased rotarod performance). KS expression was induced exclusively in a subpopulation of microglia in SOD1G93A mice, and became detectable around motoneurons in the ventral horn during the early disease phase before body weight loss. During this phase, the expression of M2 microglia markers was transiently enhanced in SOD1G93A mice, while this enhancement was attenuated in SOD1G93AGlcNAc6ST-1-/- mice. Consistent with this, M2 microglia were markedly less during the early disease phase in SOD1G93AGlcNAc6ST-1-/- mice. Moreover, KS expression in microglia was also detected in some human ALS cases. This study suggests that KS plays an indispensable, suppressive role in the early phase pathogenesis of ALS and may represent a new target for therapeutic intervention.

本文言語英語
論文番号e66969
ジャーナルPloS one
8
6
DOI
出版ステータス出版済み - 6 25 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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