Abnormal stability of wild-type p53 protein in a human lung carcinoma cell line

Motohiro Yamauchi, Keiji Suzuki, Seiji Kodama, Masami Watanabe

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

We report here that ectopically expressed wild-type p53 protein showed more than 6 times longer half-life than normal human fibroblasts in NCl-H1299, a widely used cell line derived from non-small cell lung carcinoma lacking the expression of p53 protein. We found no abnormality in the phosphorylation and ubiquitination of p53, and the expression levels of MDM2. Although proteasome activity measured in vitro was not significantly different between the tumor cell line and normal human fibroblasts, proteasome inhibitors, ALLN, MG115, and MG132, did not accumulate p53 protein in the tumor cell line, but did accumulate p53 in normal human cells. These results provide a novel mechanism, by which p53 is stabilized in tumor cells, and they suggest that a mediator should exist between ubiquitinated p53 and proteasome, which may be defective in H1299 cells.

本文言語英語
ページ(範囲)483-488
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
330
2
DOI
出版ステータス出版済み - 5 6 2005
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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