Absorption and Metabolic Behavior of Hesperidin (Rutinosylated Hesperetin) after Single Oral Administration to Sprague-Dawley Rats

Alexia M. Nectoux, Chizumi Abe, Shu Wei Huang, Naoto Ohno, Junji Tabata, Yuji Miyata, Kazunari Tanaka, Takashi Tanaka, Haruo Yamamura, Toshiro Matsui

研究成果: ジャーナルへの寄稿記事

抄録

We investigated the absorption and metabolic behavior of hesperidin (hesperetin-7-O-rutinoside) in the blood system of Sprague-Dawley rats by liquid chromatography- and matrix-assisted laser desorption ionization mass spectrometries (LC-MS and MALDI-MS). After a single oral administration of hesperidin (10 mg/kg), which was expected to be absorbed in its degraded hesperetin form, we detected intact hesperidin in the portal vein blood (tmax, 2 h) for the first time. We successfully detected glucuronized hesperidin in the circulating bloodstream, while intact hesperidin had disappeared. Further MS analyses revealed that homoeriodictyol and eriodictyol conjugates were detected in both portal and circulating blood systems. This indicated that hesperidin and/or hesperetin are susceptible to methylation and demethylation during the intestinal membrane transport process. Sulfated and glucuronized metabolites were also detected in both blood systems. In conclusion, hesperidin can enter into the circulating bloodstream in its conjugated forms, together with the conjugated forms of hesperetin, homoeriodictyol, and/or eriodictyol.

元の言語英語
ページ(範囲)9812-9819
ページ数8
ジャーナルJournal of Agricultural and Food Chemistry
67
発行部数35
DOI
出版物ステータス出版済み - 9 4 2019

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Hesperidin
hesperetin
hesperidin
oral administration
Oral Administration
Sprague Dawley Rats
Rats
rats
Blood
matrix-assisted laser desorption-ionization mass spectrometry
blood
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
blood flow
portal vein
Methylation
Liquid chromatography
rutin
Metabolites
methylation
liquid chromatography

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

これを引用

Absorption and Metabolic Behavior of Hesperidin (Rutinosylated Hesperetin) after Single Oral Administration to Sprague-Dawley Rats. / Nectoux, Alexia M.; Abe, Chizumi; Huang, Shu Wei; Ohno, Naoto; Tabata, Junji; Miyata, Yuji; Tanaka, Kazunari; Tanaka, Takashi; Yamamura, Haruo; Matsui, Toshiro.

:: Journal of Agricultural and Food Chemistry, 巻 67, 番号 35, 04.09.2019, p. 9812-9819.

研究成果: ジャーナルへの寄稿記事

Nectoux, Alexia M. ; Abe, Chizumi ; Huang, Shu Wei ; Ohno, Naoto ; Tabata, Junji ; Miyata, Yuji ; Tanaka, Kazunari ; Tanaka, Takashi ; Yamamura, Haruo ; Matsui, Toshiro. / Absorption and Metabolic Behavior of Hesperidin (Rutinosylated Hesperetin) after Single Oral Administration to Sprague-Dawley Rats. :: Journal of Agricultural and Food Chemistry. 2019 ; 巻 67, 番号 35. pp. 9812-9819.
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abstract = "We investigated the absorption and metabolic behavior of hesperidin (hesperetin-7-O-rutinoside) in the blood system of Sprague-Dawley rats by liquid chromatography- and matrix-assisted laser desorption ionization mass spectrometries (LC-MS and MALDI-MS). After a single oral administration of hesperidin (10 mg/kg), which was expected to be absorbed in its degraded hesperetin form, we detected intact hesperidin in the portal vein blood (tmax, 2 h) for the first time. We successfully detected glucuronized hesperidin in the circulating bloodstream, while intact hesperidin had disappeared. Further MS analyses revealed that homoeriodictyol and eriodictyol conjugates were detected in both portal and circulating blood systems. This indicated that hesperidin and/or hesperetin are susceptible to methylation and demethylation during the intestinal membrane transport process. Sulfated and glucuronized metabolites were also detected in both blood systems. In conclusion, hesperidin can enter into the circulating bloodstream in its conjugated forms, together with the conjugated forms of hesperetin, homoeriodictyol, and/or eriodictyol.",
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AU - Abe, Chizumi

AU - Huang, Shu Wei

AU - Ohno, Naoto

AU - Tabata, Junji

AU - Miyata, Yuji

AU - Tanaka, Kazunari

AU - Tanaka, Takashi

AU - Yamamura, Haruo

AU - Matsui, Toshiro

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