Accumulation of adenine DNA glycosylase-sensitive sites in human mitochondrial DNA

Kenichi Miyako, Chihiro Takamatsu, Shuyo Umeda, Tatsurou Tajiri, Masato Furuichi, Yusaku Nakabeppu, Mutsuo Sekiguchi, Naotaka Hamasaki, Koichiro Takeshige, Dongchon Kang

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

The mitochondrial respiratory chain inevitably produces reactive oxygen species as byproducts of aerobic ATP synthesis. Mitochondrial DNA (mtDNA), which is located close to the respiratory chain, is reported to contain much more 8-oxoguanine (8-oxoG), an oxidatively modified guanine base, than nuclear DNA. Despite such a high amount of 8-oxoG in mtDNA (1-2 8-oxoG/104 G), mtDNA is barely cleaved by an 8-oxoG DNA glycosylase or MutM, which specifically excises 8-oxoG from a C:8-oxoG pair. We find here that about half of human mtDNA molecules are cleaved by another 8-oxogG-recognizing enzyme, an adenine DNA glycosylase or MutY, which excises adenine from an A:8-oxoG pair. The cleavage sites are mapped to adenines. The calculated number of MutY-sensitive sites in mtDNA is approximately 1.4/104. This value roughly corresponds with the electrochemically measured amount of 8-oxoG in mtDNA (2.2/104), raising the possibility that 8-oxoG mainly accumulates as an A:8-oxoG pair.

元の言語英語
ページ(範囲)12326-12330
ページ数5
ジャーナルJournal of Biological Chemistry
275
発行部数16
DOI
出版物ステータス出版済み - 4 21 2000

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DNA Glycosylases
Adenine
Mitochondrial DNA
Electron Transport
Guanine
Byproducts
adenine glycosylase
Reactive Oxygen Species
Adenosine Triphosphate
Molecules
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Accumulation of adenine DNA glycosylase-sensitive sites in human mitochondrial DNA. / Miyako, Kenichi; Takamatsu, Chihiro; Umeda, Shuyo; Tajiri, Tatsurou; Furuichi, Masato; Nakabeppu, Yusaku; Sekiguchi, Mutsuo; Hamasaki, Naotaka; Takeshige, Koichiro; Kang, Dongchon.

:: Journal of Biological Chemistry, 巻 275, 番号 16, 21.04.2000, p. 12326-12330.

研究成果: ジャーナルへの寄稿記事

Miyako, K, Takamatsu, C, Umeda, S, Tajiri, T, Furuichi, M, Nakabeppu, Y, Sekiguchi, M, Hamasaki, N, Takeshige, K & Kang, D 2000, 'Accumulation of adenine DNA glycosylase-sensitive sites in human mitochondrial DNA' Journal of Biological Chemistry, 巻. 275, 番号 16, pp. 12326-12330. https://doi.org/10.1074/jbc.275.16.12326
Miyako, Kenichi ; Takamatsu, Chihiro ; Umeda, Shuyo ; Tajiri, Tatsurou ; Furuichi, Masato ; Nakabeppu, Yusaku ; Sekiguchi, Mutsuo ; Hamasaki, Naotaka ; Takeshige, Koichiro ; Kang, Dongchon. / Accumulation of adenine DNA glycosylase-sensitive sites in human mitochondrial DNA. :: Journal of Biological Chemistry. 2000 ; 巻 275, 番号 16. pp. 12326-12330.
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