Accumulation of p60 lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171

Yoichi Moroi, Yasuhiro Koga, Kazuhiko Nakamura, Masumi Ohtsu, Genki Kimura, Kikuo Nomoto

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56 lck , whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti‐p56 lck monoclonal antibody, was produced by using a 25‐amino‐acid synthetic polypeptide as the antigen, its structure corresponding to the N‐terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60 lck , and the decrease of p56 lck in human T cell leukemia virus type I (HTLV‐I)‐transformed T cell lines. Another anti‐Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C‐terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60 lck in those HTLV‐1‐transformed T cell lines. The appearance of p60 lck with the decrease of p56 lck in normal T lymphocytes after stimulation suggested the origin of p60 lck in HTLV‐I‐transformed T cells.

元の言語英語
ページ(範囲)909-915
ページ数7
ジャーナルJapanese Journal of Cancer Research
82
発行部数8
DOI
出版物ステータス出版済み - 1 1 1991

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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Monoclonal Antibodies
T-Lymphocytes
Cell Line
Complementary DNA
Peptides
Transformed Cell Line
Human T-lymphotropic virus 1
Lymphocyte Activation
Antigens
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Accumulation of p60 lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171 . / Moroi, Yoichi; Koga, Yasuhiro; Nakamura, Kazuhiko; Ohtsu, Masumi; Kimura, Genki; Nomoto, Kikuo.

:: Japanese Journal of Cancer Research, 巻 82, 番号 8, 01.01.1991, p. 909-915.

研究成果: ジャーナルへの寄稿記事

Moroi, Yoichi ; Koga, Yasuhiro ; Nakamura, Kazuhiko ; Ohtsu, Masumi ; Kimura, Genki ; Nomoto, Kikuo. / Accumulation of p60 lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171 :: Japanese Journal of Cancer Research. 1991 ; 巻 82, 番号 8. pp. 909-915.
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abstract = "The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56 lck , whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti‐p56 lck monoclonal antibody, was produced by using a 25‐amino‐acid synthetic polypeptide as the antigen, its structure corresponding to the N‐terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60 lck , and the decrease of p56 lck in human T cell leukemia virus type I (HTLV‐I)‐transformed T cell lines. Another anti‐Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C‐terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60 lck in those HTLV‐1‐transformed T cell lines. The appearance of p60 lck with the decrease of p56 lck in normal T lymphocytes after stimulation suggested the origin of p60 lck in HTLV‐I‐transformed T cells.",
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T1 - Accumulation of p60 lck in HTLV‐I‐transformed T Cell Lines Detected by an Anti‐Lck Monoclonal Antibody, MOL 171

AU - Moroi, Yoichi

AU - Koga, Yasuhiro

AU - Nakamura, Kazuhiko

AU - Ohtsu, Masumi

AU - Kimura, Genki

AU - Nomoto, Kikuo

PY - 1991/1/1

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N2 - The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56 lck , whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti‐p56 lck monoclonal antibody, was produced by using a 25‐amino‐acid synthetic polypeptide as the antigen, its structure corresponding to the N‐terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60 lck , and the decrease of p56 lck in human T cell leukemia virus type I (HTLV‐I)‐transformed T cell lines. Another anti‐Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C‐terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60 lck in those HTLV‐1‐transformed T cell lines. The appearance of p60 lck with the decrease of p56 lck in normal T lymphocytes after stimulation suggested the origin of p60 lck in HTLV‐I‐transformed T cells.

AB - The lck gene encodes a protein tyrosine kinase of nonreceptor type, p56 lck , whose expression occurs almost exclusively in T lymphocytes. MOL 171, an anti‐p56 lck monoclonal antibody, was produced by using a 25‐amino‐acid synthetic polypeptide as the antigen, its structure corresponding to the N‐terminal region deduced from the lck cDNA sequence. Immunoblot analysis with MOL 171 showed the accumulation of 60 kD form of Lck protein, p60 lck , and the decrease of p56 lck in human T cell leukemia virus type I (HTLV‐I)‐transformed T cell lines. Another anti‐Lck monoclonal antibody, MOL 294, raised by using a synthetic peptide corresponding to the C‐terminal region deduced from the lck cDNA sequence, also detected the accumulation of p60 lck in those HTLV‐1‐transformed T cell lines. The appearance of p60 lck with the decrease of p56 lck in normal T lymphocytes after stimulation suggested the origin of p60 lck in HTLV‐I‐transformed T cells.

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