Actin filaments regulate microtubule growth at the centrosome

Daisuke Inoue, Dorian Obino, Judith Pineau, Francesca Farina, Jérémie Gaillard, Christophe Guerin, Laurent Blanchoin, Ana Maria Lennon-Duménil, Manuel Théry

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抜粋

The centrosome is the main microtubule-organizing centre. It also organizes a local network of actin filaments. However, the precise function of the actin network at the centrosome is not well understood. Here, we show that increasing densities of actin filaments at the centrosome of lymphocytes are correlated with reduced amounts of microtubules. Furthermore, lymphocyte activation resulted in disassembly of centrosomal actin and an increase in microtubule number. To further investigate the direct crosstalk between actin and microtubules at the centrosome, we performed in vitro reconstitution assays based on (i) purified centrosomes and (ii) on the co-micropatterning of microtubule seeds and actin filaments. These two assays demonstrated that actin filaments constitute a physical barrier blocking elongation of nascent microtubules. Finally, we showed that cell adhesion and cell spreading lead to lower densities of centrosomal actin, thus resulting in higher microtubule growth. We therefore propose a novel mechanism, by which the number of centrosomal microtubules is regulated by cell adhesion and actin-network architecture.

元の言語英語
記事番号e99630
ジャーナルEMBO Journal
38
発行部数11
DOI
出版物ステータス出版済み - 6 3 2019

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • これを引用

    Inoue, D., Obino, D., Pineau, J., Farina, F., Gaillard, J., Guerin, C., Blanchoin, L., Lennon-Duménil, A. M., & Théry, M. (2019). Actin filaments regulate microtubule growth at the centrosome. EMBO Journal, 38(11), [e99630]. https://doi.org/10.15252/embj.201899630