Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide antigen stimulates production of chemotactic factors and inflammatory cytokines by human monocytes

Noboru Yamaguchi, Yoshihisa Yamashita, Daisuke Ikeda, Toshihiko Koga

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1α, IL-1β, IL-6, and TNF alpha (TNF-α) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1α, IL-1β, IL-6, and TNF-α, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.

元の言語英語
ページ(範囲)2563-2570
ページ数8
ジャーナルInfection and Immunity
64
発行部数7
出版物ステータス出版済み - 7 19 1996

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Aggregatibacter actinomycetemcomitans
Chemotactic Factors
Polysaccharides
Monocytes
Interleukin-1
Cytokines
Antigens
Tumor Necrosis Factor-alpha
Interleukin-8
Interleukin-6
Chemokine CCL2
Messenger RNA
Polymyxin B
Serogroup
Chromatography
Leukocytes
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

これを引用

Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide antigen stimulates production of chemotactic factors and inflammatory cytokines by human monocytes. / Yamaguchi, Noboru; Yamashita, Yoshihisa; Ikeda, Daisuke; Koga, Toshihiko.

:: Infection and Immunity, 巻 64, 番号 7, 19.07.1996, p. 2563-2570.

研究成果: ジャーナルへの寄稿記事

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abstract = "Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1α, IL-1β, IL-6, and TNF alpha (TNF-α) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1α, IL-1β, IL-6, and TNF-α, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.",
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AU - Yamaguchi, Noboru

AU - Yamashita, Yoshihisa

AU - Ikeda, Daisuke

AU - Koga, Toshihiko

PY - 1996/7/19

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N2 - Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1α, IL-1β, IL-6, and TNF alpha (TNF-α) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1α, IL-1β, IL-6, and TNF-α, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.

AB - Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1α, IL-1β, IL-6, and TNF alpha (TNF-α) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1α, IL-1β, IL-6, and TNF-α, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.

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