Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide antigen stimulates production of chemotactic factors and inflammatory cytokines by human monocytes

Noboru Yamaguchi, Yoshihisa Yamashita, Daisuke Ikeda, Toshihiko Koga

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had almost no effect on the release of monocyte chemotactic factor, but a monoclonal antibody against SPA markedly inhibited it. Human monocytes stimulated with SPA exhibited the increased mRNA expression of monocyte chemoattractant protein 1 (MCP-1) and a neutrophil chemotactic factor, interleukin-8 (IL-8). On the other hand, SPA induced the release of IL-1, IL-6, and tumor necrosis factor (TNF) and enhanced the expression of IL-1α, IL-1β, IL-6, and TNF alpha (TNF-α) mRNAs. Human monocytes expressed MCP-1 and IL-8 mRNAs when stimulated by human recombinant IL-1α, IL-1β, IL-6, and TNF-α, suggesting that these inflammatory cytokines induced by SPA might participate in the production of chemotactic factors in human monocytes.

本文言語英語
ページ(範囲)2563-2570
ページ数8
ジャーナルInfection and Immunity
64
7
DOI
出版ステータス出版済み - 1996

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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