Activation of ataxia telangiectasia-mutated DNA damage checkpoint signal transduction elicited by herpes simplex virus infection

Noriko Shirata, Ayumi Kudoh, Tohru Daikoku, Yasutoshi Tatsumi, Masatoshi Fujita, Tohru Kiyono, Yutaka Sugaya, Hiroki Isomura, Kanji Ishizaki, Tatsuya Tsurumi

研究成果: ジャーナルへの寄稿記事

102 引用 (Scopus)

抄録

Eukaryotic cells are equipped with machinery to monitor and repair damaged DNA. Herpes simplex virus (HSV) DNA replication occurs at discrete sites in nuclei, the replication compartment, where viral replication proteins cluster and synthesize a large amount of viral DNA. In the present study, HSV infection was found to elicit a cellular DNA damage response, with activation of the ataxia-telangiectasia-mutated (ATM) signal transduction pathway, as observed by autophosphorylation of ATM and phosphorylation of multiple downstream targets including Nbs1, Chk2, and p53, while infection with a UV-inactivated virus or with a replication-defective virus did not. Activated ATM and the DNA damage sensor MRN complex composed of Mre11, Rad50, and Nbs1 were recruited and retained at sites of viral DNA replication, probably recognizing newly synthesized viral DNAs as abnormal DNA structures. These events were not observed in ATM-deficient cells, indicating ATM dependence. In Nbs1-deficient cells, HSV infection induced an ATM DNA damage response that was delayed, suggesting a functional MRN complex requirement for efficient ATM activation. However, ATM silencing had no effect on viral replication in 293T cells. Our data open up an interesting question of how the virus is able to complete its replication, although host cells activate ATM checkpoint signaling in response to the HSV infection.

元の言語英語
ページ(範囲)30336-30341
ページ数6
ジャーナルJournal of Biological Chemistry
280
発行部数34
DOI
出版物ステータス出版済み - 8 26 2005
外部発表Yes

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Ataxia Telangiectasia
Signal transduction
Virus Diseases
Simplexvirus
Viruses
DNA Damage
Signal Transduction
Chemical activation
DNA
Viral DNA
DNA Replication
Phosphorylation
Defective Viruses
Machinery
HEK293 Cells
Eukaryotic Cells
Viral Proteins
Virus Replication
Repair
DNA Repair

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Activation of ataxia telangiectasia-mutated DNA damage checkpoint signal transduction elicited by herpes simplex virus infection. / Shirata, Noriko; Kudoh, Ayumi; Daikoku, Tohru; Tatsumi, Yasutoshi; Fujita, Masatoshi; Kiyono, Tohru; Sugaya, Yutaka; Isomura, Hiroki; Ishizaki, Kanji; Tsurumi, Tatsuya.

:: Journal of Biological Chemistry, 巻 280, 番号 34, 26.08.2005, p. 30336-30341.

研究成果: ジャーナルへの寄稿記事

Shirata, N, Kudoh, A, Daikoku, T, Tatsumi, Y, Fujita, M, Kiyono, T, Sugaya, Y, Isomura, H, Ishizaki, K & Tsurumi, T 2005, 'Activation of ataxia telangiectasia-mutated DNA damage checkpoint signal transduction elicited by herpes simplex virus infection', Journal of Biological Chemistry, 巻. 280, 番号 34, pp. 30336-30341. https://doi.org/10.1074/jbc.M500976200
Shirata, Noriko ; Kudoh, Ayumi ; Daikoku, Tohru ; Tatsumi, Yasutoshi ; Fujita, Masatoshi ; Kiyono, Tohru ; Sugaya, Yutaka ; Isomura, Hiroki ; Ishizaki, Kanji ; Tsurumi, Tatsuya. / Activation of ataxia telangiectasia-mutated DNA damage checkpoint signal transduction elicited by herpes simplex virus infection. :: Journal of Biological Chemistry. 2005 ; 巻 280, 番号 34. pp. 30336-30341.
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