Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide

Koei Kawakami, Taiki Urakawa, Yasuhiro Oda, Yoshio Iwai

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

Background: Sol-gel entrapment of lipases is usually performed in an aqueous solution. A novel method of sol-gel coating of lipasde in supercritical carbon dioxide (SC-CO2) is proposed. Results: Crude lipase powder (Rhizopus oryzae) coatedwith hydrophobic silicates, derived from dimethyldimethoxysilane and tetramethoxysilane in SC-CO2 at 35 °C and 15 MPa, exhibited 5-7 times higher esterification activity than that prepared via an aqueous sol-gel route. Lipase immobilized in amethyl-substituted silica monolithwas also highly activated by sol-gel coating using the same silica precursors in SC-CO2. Conclusion: Sol-gel coating in SC-CO2, of lipases in powder and immobilized forms with hydrophobic alkyl-substituted silicates provides an efficient tool for the enhancement of enzymatic activities in non-aqueous media.

本文言語英語
ページ(範囲)1412-1417
ページ数6
ジャーナルJournal of Chemical Technology and Biotechnology
84
9
DOI
出版ステータス出版済み - 10月 5 2009

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 化学工学(全般)
  • 再生可能エネルギー、持続可能性、環境
  • 燃料技術
  • 廃棄物管理と処理
  • 汚染
  • 有機化学
  • 無機化学

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