TY - JOUR
T1 - Activation of NF-κB promotes the transition of large, CD43+ pre-B cells to small, CD43- pre-B cells
AU - Jimi, Eijiro
AU - Phillips, Roderick J.
AU - Rincon, Mercedes
AU - Voll, Reinhard
AU - Karasuyama, Hajime
AU - Flavell, Richard
AU - Ghosh, Sankar
N1 - Funding Information:
We would like to thank Crystal Bussey and Iris Douglas for technical help. This work was supported by a grant from NIH (R37-AI33443).
PY - 2005/6
Y1 - 2005/6
N2 - The regulation of the transcription factor nuclear factor-κB (NF-κB) during B-cell development was examined using cells isolated from the bone marrow of transgenic mice expressing a κB luciferase reporter gene. The results indicate that the highest level of NF-κB activity is present in cells expressing the pre-B-cell receptor. Furthermore, cross-linking of Igβ on CD43+ pre-B cells is able to activate NF-κB in recombination-activating gene 1-deficient mice, preceding their further differentiation into CD43- pre-B cells. Expression of a dominant negative form of IκBα using a transgenic approach or by retroviral infection leads to a reduction in the number of CD43+ pre-B cells. These data therefore indicate that activation of NF-κB in CD43+ pre-B cells, as a result of signaling by the pre-B-cell receptor, facilitates the continued development of large, CD43+ pre-B cells into small CD43- pre-B cells.
AB - The regulation of the transcription factor nuclear factor-κB (NF-κB) during B-cell development was examined using cells isolated from the bone marrow of transgenic mice expressing a κB luciferase reporter gene. The results indicate that the highest level of NF-κB activity is present in cells expressing the pre-B-cell receptor. Furthermore, cross-linking of Igβ on CD43+ pre-B cells is able to activate NF-κB in recombination-activating gene 1-deficient mice, preceding their further differentiation into CD43- pre-B cells. Expression of a dominant negative form of IκBα using a transgenic approach or by retroviral infection leads to a reduction in the number of CD43+ pre-B cells. These data therefore indicate that activation of NF-κB in CD43+ pre-B cells, as a result of signaling by the pre-B-cell receptor, facilitates the continued development of large, CD43+ pre-B cells into small CD43- pre-B cells.
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U2 - 10.1093/intimm/dxh263
DO - 10.1093/intimm/dxh263
M3 - Article
C2 - 15908447
AN - SCOPUS:26444604518
VL - 17
SP - 815
EP - 825
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 6
ER -