Activation of P2X7 receptors induces CCL3 production in microglial cells through transcription factor NFAT

Ayako Kataoka, Hidetoshi Tozaki-Saitoh, Yui Koga, Makoto Tsuda, Kazuhide Inoue

研究成果: Contribution to journalArticle査読

95 被引用数 (Scopus)

抄録

Microglia are implicated as a source of diverse proinflammatory factors in the CNS. Extracellular nucleotides are well known to be potent activators of glial cells and trigger the release of cytokines from microglia through purinergic receptors. However, little is known about the role of purinoceptors in microglial chemokine release. In this study, we found that high concentrations of ATP evoked release of CC-chemokine ligand 3 (CCL3)/macrophage inflammatory protein-1α from MG-5 cells, a mouse microglial cell line, and rapid up-regulation of CCL3 mRNA was elicited within 30 min of ATP stimulation. The release of CCL3 was also stimulated by 2′- and 3′-O-(4- benzoylbenzoyl) ATP, an agonist of P2X7 receptors. Brilliant Blue G, an antagonist of P2X7 receptors, strongly inhibited this ATP-induced CCL3 release. Similar pharmacological profile was observed in primary microglia. In MG-5 cells, ATP caused de-phosphorylation and nuclear translocation of the transcription factor nuclear factor of activated T cells (NFAT). ATP-induced NFAT de-phosphorylation was also dependent on P2X7 receptor activation. Furthermore, ATP-induced CCL3 release and production were prevented by a selective inhibitor of NFAT. Taken together, the results of this study demonstrate an involvement of NFAT in the mechanism underlying P2X7 receptor-mediated CCL3 release.

本文言語英語
ページ(範囲)115-125
ページ数11
ジャーナルJournal of Neurochemistry
108
1
DOI
出版ステータス出版済み - 1 2009

All Science Journal Classification (ASJC) codes

  • 生化学
  • 細胞および分子神経科学

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