TY - JOUR
T1 - Activation of protease by sol-gel entrapment into organically modified hybrid silicates
AU - Furukawa, Shin Ya
AU - Ono, Tsutomu
AU - Ijima, Hiroyuki
AU - Kawakami, Koei
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.
AB - To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.
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U2 - 10.1023/A:1013876313806
DO - 10.1023/A:1013876313806
M3 - Article
AN - SCOPUS:0036160284
SN - 0141-5492
VL - 24
SP - 13
EP - 16
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 1
ER -