Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development

Yusuke Ohnishi, Yasushi Totoki, Atsushi Toyoda, Toshiaki Watanabe, Yasuhiro Yamamoto, Katsushi Tokunaga, Yoshiyuki Sakaki, Hiroyuki Sasaki, Hirohiko Hohjoh

    研究成果: Contribution to journalArticle査読

    16 被引用数 (Scopus)

    抄録

    Small RNAs derived from repetitive sequences appear to play essential roles in mammalian gametogenesis and early development. In this study we focused on the short interspersed nucleotide element B1 (SINE/B1) small RNAs, which were zygotically expressed in pre-implantation mouse embryos; and we investigated whether the SINE/B1 small RNAs played an active role in gene silencing during early mouse development. The results indicated that the level of silencing activity involving the SINE/B1 small RNAs as mediators was significantly reduced in Dicer-knockdown mouse embryos. In addition, when the SINE/B1 small RNAs were mapped to a full-length SINE/B1 sequence, phase-distribution of the small RNAs appeared, suggesting possible enzymatic involvement. Therefore, our present study suggested that the zygotically expressed SINE/B1 small RNAs in pre-implantation mouse embryos contain active small RNAs, which were presumably processed by Dicer and involved in gene silencing.

    本文言語英語
    ページ(範囲)903-909
    ページ数7
    ジャーナルMolecular biology reports
    39
    2
    DOI
    出版ステータス出版済み - 2 2012

    All Science Journal Classification (ASJC) codes

    • 分子生物学
    • 遺伝学

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