Activin receptor mRNA is expressed early in Xenopus embryogenesis and the level of the expression affects the body axis formation

Mariko Kondo, Kosuke Tashiro, Gen Fujii, Misaki Asano, Ryutaro Miyoshi, Ryutaro Yamada, Masami Muramatsu, Koichiro Shiokawa

研究成果: Contribution to journalArticle査読

74 被引用数 (Scopus)

抄録

Activin is a member of the transforming growth factor β (TGF-β) and possesses various activities in cellular control phenomena. During Xenopus embryonic development, activin is thought to act as a natural mesoderm-inducing factor. We isolated here the Xenopus activin receptor cDNA from Xenopus tadpole cDNA library and examined the expression of the Xenopus activin receptor gene during the course of early embryonic development. The Xenopus activin receptor has an 87% homology at the level of deduced amino acid sequence with the mouse activin receptor, and using the cDNA obtained, three bands of mRNA with different lengths were detected in Xenopus embryos throughout early embryogenesis. We synthesized activin receptor mRNA in vitro and tested the effect of the injection of the mRNA into Xenopus fertllized eggs on subsequent development. When the synthetic mRNA was injected into uncleaved fertllized eggs, embryos with reduced trunk structure were formed. However, when the mRNA was injected into the ventral blastomeres at the 16-cell stage, embryos with a secondary body axis were formed. These results indicate the importance of the function of activin receptor in the reguiatory mechanism for body axis formation.

本文言語英語
ページ(範囲)684-690
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
181
2
DOI
出版ステータス出版済み - 12 16 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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