Adaptor polymerase chain reaction for single molecule amplification

Michihiko Nakano, Jun Komatsu, Hirofumi Kurita, Hachiro Yasuda, Shinji Katsura, Akira Mizuno

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.

元の言語英語
ページ(範囲)216-218
ページ数3
ジャーナルJournal of Bioscience and Bioengineering
100
発行部数2
DOI
出版物ステータス出版済み - 1 1 2005
外部発表Yes

Fingerprint

Polymerase chain reaction
Amplification
DNA
Polymerase Chain Reaction
Molecules
Oligonucleotides
Plasmids
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Adaptor polymerase chain reaction for single molecule amplification. / Nakano, Michihiko; Komatsu, Jun; Kurita, Hirofumi; Yasuda, Hachiro; Katsura, Shinji; Mizuno, Akira.

:: Journal of Bioscience and Bioengineering, 巻 100, 番号 2, 01.01.2005, p. 216-218.

研究成果: ジャーナルへの寄稿記事

Nakano, M, Komatsu, J, Kurita, H, Yasuda, H, Katsura, S & Mizuno, A 2005, 'Adaptor polymerase chain reaction for single molecule amplification', Journal of Bioscience and Bioengineering, 巻. 100, 番号 2, pp. 216-218. https://doi.org/10.1263/jbb.100.216
Nakano, Michihiko ; Komatsu, Jun ; Kurita, Hirofumi ; Yasuda, Hachiro ; Katsura, Shinji ; Mizuno, Akira. / Adaptor polymerase chain reaction for single molecule amplification. :: Journal of Bioscience and Bioengineering. 2005 ; 巻 100, 番号 2. pp. 216-218.
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