Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage

Toshihiko Fukuda, Kaustav Majumder, Hua Zhang, Toshiro Matsui, Yoshinori Mine

研究成果: ジャーナルへの寄稿学術誌査読

13 被引用数 (Scopus)

抄録

It has previously demonstrated that adenine (6-amino-6H-purine) exhibited a vasoactive effect in contracted aorta. The adenine receptor (AdeR) has recently been identified as a G protein coupled purinergic receptor in diverse organs. It has been suggested that adenine acts as a signaling molecule through the activation of AdeR. The present study investigated the effect of adenine on endotoxin-stimulated mouse macrophages (RAW 264.7 cells) and focused on the AdeR signaling pathway. RAW 264.7 cells were treated with adenine and stimulated with lipopolysaccharide (LPS, Toll-like receptor 4 agonist) from Escherichia coli. Adenine inhibited tumor necrosis factor (TNF)-α secretion in RAW 264.7 cells. The inhibition by adenine was significantly attenuated in the presence of either adenyl cyclase (AC) or protein kinase A (PKA) inhibitors. Furthermore, adenine restored AdeR gene expression. These results firstly demonstrated that adenine acts as an anti-endotoxin compounds through a AdeR/AC/PKA signaling cascade.

本文言語英語
ページ(範囲)235-239
ページ数5
ジャーナルJournal of Functional Foods
28
DOI
出版ステータス出版済み - 1月 1 2017

!!!All Science Journal Classification (ASJC) codes

  • 食品科学
  • 医学(その他)
  • 栄養および糖尿病

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