Adenovirus-mediated ectopic expression of Msx2 in even-numbered rhombomeres induces apoptotic elimination of cranial neural crest cells in ovo

Katsu Takahashi, Glen H. Nuckolls, Osamu Tanaka, Ichiro Semba, Ichiro Takahashi, Ralph Dashner, Lillian Shum, Harold C. Slavkin

研究成果: ジャーナルへの寄稿学術誌査読

53 被引用数 (Scopus)

抄録

Distinct cranial neural crest-derived cell types (a number of neuronal as well as non-neuronal cell lineages) are generated at characteristic times and positions in the rhombomeres of the hindbrain in developing vertebrate embryos. To examine this developmental process, we developed a novel strategy designed to test the efficacy of gain-of-function Msx2 expression within rhombomeres in ovo prior to the emigration of cranial neural crest cells (CNCC). Previous studies indicate that CNCC from odd-numbered rhombomeres (r3 and r5) undergo apoptosis in response to exogenous BMP4. We provide evidence that targeted infection in ovo using adenovirus containing Msx2 and a reporter molecule indicative of translation can induce apoptosis in either even- or odd-numbered rhombomeres. Furthermore, infected lacZ-control explants indicated that CNCC emigrated, and that 20% of these cells were double positive for crest cell markers HNK-1 and β-gal. In contrast, there were no HNK-1 and Msx2 double positive cells emigrating from Msx2 infected explants. These results support the hypothesis that apoptotic elimination of CNCC can be induced by 'gain-of-function' Msx2 expression in even-numbered rhombomeres. These inductive interactions involve qualitative, quantitative, positional and temporal differences in TGF-β-related signals, Msx2 expression and other transcriptional control.

本文言語英語
ページ(範囲)1627-1635
ページ数9
ジャーナルDevelopment
125
9
出版ステータス出版済み - 1998
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 発生生物学

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