TY - JOUR
T1 - Adenovirus-mediated gene transfer into the brain stem to examine cardiovascular function
T2 - Role of nitric oxide and Rho-kinase
AU - Hirooka, Yoshitaka
N1 - Funding Information:
These studies were supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture (C11670689, C13670721) and by a Grant for Research on Autonomic Nervous System and Hypertension from Kimura Memorial Heart Foundation/Pfizer Pharmaceuticals, Inc. Particularly, I thank Drs. Koji Sakai, Takuya Kishi, Koji Ito, Hiroaki Shimokawa, and Akira Takeshita at our department, and Dr. Kozo Kaibuchi at the Department of Cell Pharmacology, Nagoya University Graduate School of Medicine, for conducting this series of studies. I also thank Drs. Donald D. Heistad and Beverly L. Davidson (The University of Iowa Gene Transfer Vector Core, supported by National Institutes of Health grants and the Carver Foundation), for the preparation of vectors.
PY - 2004
Y1 - 2004
N2 - The central nervous system plays an important role in the regulation of blood pressure via the sympathetic nervous system. Abnormal regulation of the sympathetic nerve activity is involved in the pathophysiology of hypertension. In particular, the brain stem, including the nucleus tractus solitarii (NTS) and the rostral ventrolateral medulla (RVLM), is a key site that controls and maintains blood pressure via the sympathetic nervous system. Nitric oxide (NO) is a unique molecule that influences sympathetic nerve activity. Rho-kinase is a downstream effector of the small GTPase, Rho, and is implicated in various cellular functions. We developed a technique to transfer adenovirus vectors encoding endothelial nitric oxide synthase and dominant-negative Rho-kinase into the NTS or the RVLM of rats in vivo. We applied this technique to hypertensive rats to explore the physiological significance of NO and Rho-kinase.
AB - The central nervous system plays an important role in the regulation of blood pressure via the sympathetic nervous system. Abnormal regulation of the sympathetic nerve activity is involved in the pathophysiology of hypertension. In particular, the brain stem, including the nucleus tractus solitarii (NTS) and the rostral ventrolateral medulla (RVLM), is a key site that controls and maintains blood pressure via the sympathetic nervous system. Nitric oxide (NO) is a unique molecule that influences sympathetic nerve activity. Rho-kinase is a downstream effector of the small GTPase, Rho, and is implicated in various cellular functions. We developed a technique to transfer adenovirus vectors encoding endothelial nitric oxide synthase and dominant-negative Rho-kinase into the NTS or the RVLM of rats in vivo. We applied this technique to hypertensive rats to explore the physiological significance of NO and Rho-kinase.
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U2 - 10.1016/j.pbiomolbio.2003.11.008
DO - 10.1016/j.pbiomolbio.2003.11.008
M3 - Review article
C2 - 14769438
AN - SCOPUS:1242276572
VL - 84
SP - 233
EP - 249
JO - Progress in Biophysics and Molecular Biology
JF - Progress in Biophysics and Molecular Biology
SN - 0079-6107
IS - 2-3
ER -