Adenovirus-mediated gene transfer into the NTS in conscious rats: A new approach to examining the central control of cardiovascular regulation

Yoshitaka Hirooka, Koji Sakai, Takuya Kishi, Akira Takeshita

    研究成果: Contribution to journalArticle査読

    25 被引用数 (Scopus)

    抄録

    The nucleus tractus solitarii (NTS) is an important site for the regulation of sympathetic nerve activity. It receives the signals through afferent fibers from arterial baroreceptors, chemoreceptors, cardiopulmonary receptors, and other visceral receptors. Many studies have examined the role of nitric oxide (NO) in the NTS in cardiovascular regulation. However, most of these studies were conducted in an acute state with anesthesia. We have developed a novel technique of endothelial nitric oxide synthase (eNOS) gene transfer into the NTS in vivo. Adenovirus vectors encoding either the β-galactosidase gene (Adβgal) or the endothelial nitric oxide synthase gene (Ad-eNOS) gene were transfected into the NTS. In the Adβgal-treated rats, the local expression of β-galactosidase was confirmed by X-Gal staining, and β-galactosidase activity was quantified using a colorimetric assay. In the AdeNOS-treated rats, the local expression of eNOS protein was confirmed by immunohistochemistry, and eNOS production was measured by in vivo microdialysis. Blood pressure and heart rate were monitored by a radiotelemetry system in a conscious state. The expression of each gene was observed from day 5 to day 10 after the gene transfer. In the AdeNOS-treated rats, blood pressure and heart rate significantly decreased from day 5 to day 10, and then thereafter gradually recovered over time. Our method may be useful in examining the local effect of a particular substance produced by a specific gene in the brain on cardiovascular function.

    本文言語英語
    ページ(範囲)197-205
    ページ数9
    ジャーナルAnnals of the New York Academy of Sciences
    940
    DOI
    出版ステータス出版済み - 2001

    All Science Journal Classification (ASJC) codes

    • 神経科学(全般)
    • 生化学、遺伝学、分子生物学(全般)
    • 科学史および科学哲学

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