Adhesion attenuates respiratory burst induced by different modes of triggering in resting or LPS-primed neutrophils

Terukazu Sanui, Masaaki Takeshita, Takao Fukuda, Urara Tanaka, Rehab Alshargabi, Yoshitomi Aida, Fusanori Nishimura

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The effects of adherence on neutrophil superoxide anion (O2) generation triggered by surface, soluble ligand, or adherence were studied. Resting-neutrophils adhered to the uncoated tubes resulting in O2 generation, but not on plasma-, fibrinogen-, vitronectin-, fibronectin-, laminin-, collagen-, or poly HEMA-coated surfaces. Enhanced N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated O2 generation by LPS-primed-neutrophils was induced by the incubation on plasma, fibrinogen, vitronectin, fibronectin, or laminin in the absence of Mg2+. In the presence of Mg2+, this response was observed in cells on collagen or poly HEMA. LPS-primed-neutrophils adhered to uncoated, BSA- or IgG-coated tubes and did not respond to fMLP, indicating that the fMLP-response of LPS-primed-neutrophils was suppressed by adherence. Upon incubation on plasma, fibrinogen, vitronectin, fibronectin in the presence of Mg2+, LPS-primed-neutrophils showed O2 generation. Upon incubation on collagen or poly HEMA, the primed-neutrophils neither generated O2 nor adhered. We found that O2 response of LPS-primed-neutrophils was attenuated depending on the time of exposure to plasma-coated surface. This attenuation was evident on plasma or fibrinogen, but not on collagen in the presence of Mg2+, indicating that O2 generation by LPS-primed-neutrophils was attenuated dependent on adherence but not on Mg2+. Thus, adhesion attenuated the O2 generation triggered by both soluble (fMLP) and insoluble (surface) stimuli.

元の言語英語
ページ(範囲)865-871
ページ数7
ジャーナルImmunobiology
222
発行部数8-9
DOI
出版物ステータス出版済み - 8 2017

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Respiratory Burst
Neutrophils
methionyl-leucyl-phenylalanine
Vitronectin
Fibrinogen
Collagen
Fibronectins
Laminin
N-Formylmethionine Leucyl-Phenylalanine
Superoxides
Immunoglobulin G
Ligands

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Hematology

これを引用

Adhesion attenuates respiratory burst induced by different modes of triggering in resting or LPS-primed neutrophils. / Sanui, Terukazu; Takeshita, Masaaki; Fukuda, Takao; Tanaka, Urara; Alshargabi, Rehab; Aida, Yoshitomi; Nishimura, Fusanori.

:: Immunobiology, 巻 222, 番号 8-9, 08.2017, p. 865-871.

研究成果: ジャーナルへの寄稿記事

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title = "Adhesion attenuates respiratory burst induced by different modes of triggering in resting or LPS-primed neutrophils",
abstract = "The effects of adherence on neutrophil superoxide anion (O2−) generation triggered by surface, soluble ligand, or adherence were studied. Resting-neutrophils adhered to the uncoated tubes resulting in O2− generation, but not on plasma-, fibrinogen-, vitronectin-, fibronectin-, laminin-, collagen-, or poly HEMA-coated surfaces. Enhanced N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated O2− generation by LPS-primed-neutrophils was induced by the incubation on plasma, fibrinogen, vitronectin, fibronectin, or laminin in the absence of Mg2+. In the presence of Mg2+, this response was observed in cells on collagen or poly HEMA. LPS-primed-neutrophils adhered to uncoated, BSA- or IgG-coated tubes and did not respond to fMLP, indicating that the fMLP-response of LPS-primed-neutrophils was suppressed by adherence. Upon incubation on plasma, fibrinogen, vitronectin, fibronectin in the presence of Mg2+, LPS-primed-neutrophils showed O2− generation. Upon incubation on collagen or poly HEMA, the primed-neutrophils neither generated O2− nor adhered. We found that O2− response of LPS-primed-neutrophils was attenuated depending on the time of exposure to plasma-coated surface. This attenuation was evident on plasma or fibrinogen, but not on collagen in the presence of Mg2+, indicating that O2− generation by LPS-primed-neutrophils was attenuated dependent on adherence but not on Mg2+. Thus, adhesion attenuated the O2− generation triggered by both soluble (fMLP) and insoluble (surface) stimuli.",
author = "Terukazu Sanui and Masaaki Takeshita and Takao Fukuda and Urara Tanaka and Rehab Alshargabi and Yoshitomi Aida and Fusanori Nishimura",
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T1 - Adhesion attenuates respiratory burst induced by different modes of triggering in resting or LPS-primed neutrophils

AU - Sanui, Terukazu

AU - Takeshita, Masaaki

AU - Fukuda, Takao

AU - Tanaka, Urara

AU - Alshargabi, Rehab

AU - Aida, Yoshitomi

AU - Nishimura, Fusanori

PY - 2017/8

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N2 - The effects of adherence on neutrophil superoxide anion (O2−) generation triggered by surface, soluble ligand, or adherence were studied. Resting-neutrophils adhered to the uncoated tubes resulting in O2− generation, but not on plasma-, fibrinogen-, vitronectin-, fibronectin-, laminin-, collagen-, or poly HEMA-coated surfaces. Enhanced N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated O2− generation by LPS-primed-neutrophils was induced by the incubation on plasma, fibrinogen, vitronectin, fibronectin, or laminin in the absence of Mg2+. In the presence of Mg2+, this response was observed in cells on collagen or poly HEMA. LPS-primed-neutrophils adhered to uncoated, BSA- or IgG-coated tubes and did not respond to fMLP, indicating that the fMLP-response of LPS-primed-neutrophils was suppressed by adherence. Upon incubation on plasma, fibrinogen, vitronectin, fibronectin in the presence of Mg2+, LPS-primed-neutrophils showed O2− generation. Upon incubation on collagen or poly HEMA, the primed-neutrophils neither generated O2− nor adhered. We found that O2− response of LPS-primed-neutrophils was attenuated depending on the time of exposure to plasma-coated surface. This attenuation was evident on plasma or fibrinogen, but not on collagen in the presence of Mg2+, indicating that O2− generation by LPS-primed-neutrophils was attenuated dependent on adherence but not on Mg2+. Thus, adhesion attenuated the O2− generation triggered by both soluble (fMLP) and insoluble (surface) stimuli.

AB - The effects of adherence on neutrophil superoxide anion (O2−) generation triggered by surface, soluble ligand, or adherence were studied. Resting-neutrophils adhered to the uncoated tubes resulting in O2− generation, but not on plasma-, fibrinogen-, vitronectin-, fibronectin-, laminin-, collagen-, or poly HEMA-coated surfaces. Enhanced N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated O2− generation by LPS-primed-neutrophils was induced by the incubation on plasma, fibrinogen, vitronectin, fibronectin, or laminin in the absence of Mg2+. In the presence of Mg2+, this response was observed in cells on collagen or poly HEMA. LPS-primed-neutrophils adhered to uncoated, BSA- or IgG-coated tubes and did not respond to fMLP, indicating that the fMLP-response of LPS-primed-neutrophils was suppressed by adherence. Upon incubation on plasma, fibrinogen, vitronectin, fibronectin in the presence of Mg2+, LPS-primed-neutrophils showed O2− generation. Upon incubation on collagen or poly HEMA, the primed-neutrophils neither generated O2− nor adhered. We found that O2− response of LPS-primed-neutrophils was attenuated depending on the time of exposure to plasma-coated surface. This attenuation was evident on plasma or fibrinogen, but not on collagen in the presence of Mg2+, indicating that O2− generation by LPS-primed-neutrophils was attenuated dependent on adherence but not on Mg2+. Thus, adhesion attenuated the O2− generation triggered by both soluble (fMLP) and insoluble (surface) stimuli.

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JO - Immunobiology

JF - Immunobiology

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