Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans

Noboru Yamaguchi, Toshio Kukita, Yin Ji Li, Jose Guillermo Martinez Argueta, Toshiyuki Saito, Shigemasa Hanazawa, Yoshihisa Yamashita

研究成果: ジャーナルへの寄稿記事

81 引用 (Scopus)

抄録

Previous epidemiologic studies have suggested that periodontal disease is closely related to obesity and glucose tolerance. As the level of adiponectin, an adipocyte-derived cytokine, in plasma had been reported to decrease in obese and type 2 diabetes patients, we explored the role of adiponectin in the etiology of periodontitis using the D clone of RAW264, a clone that exhibits highly efficient osteoclast formation, to determine whether adiponectin acts as a regulatory molecule in osteoclast formation stimulated by lipopolysaccharide of periodontopathic bacteria. We observed that adiponectin acted as a potent inhibitor of osteoclast formation stimulated by Toll-like receptor 4 (TLR4) ligand and receptor activator of NF-κB ligand (RANKL). Because NF-κB is an important transcription factor in osteoclast formation, we examined the effect of adiponectin on its transcriptional activity. A luciferase assay showed that adiponectin was able to inhibit the TLR4-mediated NF-κB activity in RAW264 cells. In addition, we observed that the cytokine was actually able to inhibit TLR4-mediated expression of the gene for inducible nitric oxide synthase and production of nitric oxide in the cells. These observations strongly suggest that adiponectin may function as a negative regulator of lipopolysaccharide/RANKL-mediated osteoclast formation in periodontal disease.

元の言語英語
ページ(範囲)28-34
ページ数7
ジャーナルFEMS Immunology and Medical Microbiology
49
発行部数1
DOI
出版物ステータス出版済み - 2 1 2007

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Aggregatibacter actinomycetemcomitans
Adiponectin
Osteoclasts
Lipopolysaccharides
Toll-Like Receptor 4
Periodontal Diseases
Clone Cells
Cytokines
Ligands
Periodontitis
Nitric Oxide Synthase Type II
Luciferases
Adipocytes
Type 2 Diabetes Mellitus
Epidemiologic Studies
Nitric Oxide
Transcription Factors
Obesity
Bacteria
Gene Expression

All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology
  • Infectious Diseases

これを引用

Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans. / Yamaguchi, Noboru; Kukita, Toshio; Li, Yin Ji; Martinez Argueta, Jose Guillermo; Saito, Toshiyuki; Hanazawa, Shigemasa; Yamashita, Yoshihisa.

:: FEMS Immunology and Medical Microbiology, 巻 49, 番号 1, 01.02.2007, p. 28-34.

研究成果: ジャーナルへの寄稿記事

Yamaguchi, Noboru ; Kukita, Toshio ; Li, Yin Ji ; Martinez Argueta, Jose Guillermo ; Saito, Toshiyuki ; Hanazawa, Shigemasa ; Yamashita, Yoshihisa. / Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans. :: FEMS Immunology and Medical Microbiology. 2007 ; 巻 49, 番号 1. pp. 28-34.
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