Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells

Marie Fukushima, Munechika Enjoji, Motoyuki Kohjima, Rie Sugimoto, Satoshi Ohta, Kazuhiro Kotoh, Masami Kuniyoshi, Kunihisa Kobayashi, Minako Imamura, Toyoshi Inoguchi, Makoto Nakamuta, Hajime Nawata

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

The function of adipose differentiation-related protein (ADRP) is known to be the uptake of long-chain fatty acids and formation of lipid droplets in lipid-accumulating cells. We hypothesized that ADRP might stimulate activated hepatic stellate cells (HSCs) to accumulate lipids, resulting in their transition to the quiescent state. In this study, cultured HSCs in fifth passages isolated from rat were infected by adenovirus vector expressing ADRP (Ad.GFP-ADRP), and morphologic and functional changes were evaluated in comparison with control HSCs infected by recombinant adenovirus-expressing β-galactosidase (Ad.LacZ). In Ad.GFP-ADRP-infected cells only, many tiny lipid droplets were apparent in the cytoplasm, while the outline of the cells was not changed. The ADRP was detected around the lipid droplets. In HSCs with intracellular actin filaments, the staining pattern of the filaments before and after infection with Ad.GFP-ADRP or Ad.LacZ did not differ. The cell proliferation rate was not influenced by infection with Ad.LacZ or Ad.GFP-ADRP. Type I collagen secretion from cells overexpressing ADRP was not significantly different from that of Ad.LacZ-infected cells. In our in vitro study, ADRP overexpression induced the formation of cytoplasmic lipid droplets in activated HSCs but could not convert other characteristics of the activated form into those of the quiescent form.

元の言語英語
ページ(範囲)321-324
ページ数4
ジャーナルIn Vitro Cellular and Developmental Biology - Animal
41
発行部数10
DOI
出版物ステータス出版済み - 11 1 2005

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Hepatic Stellate Cells
Lipids
Adenoviridae
Perilipin-2
Lipid Droplets
Galactosidases
Collagen Type I
Infection
Actin Cytoskeleton
Cultured Cells
Cytoplasm
Fatty Acids
Cell Proliferation
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

これを引用

Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells. / Fukushima, Marie; Enjoji, Munechika; Kohjima, Motoyuki; Sugimoto, Rie; Ohta, Satoshi; Kotoh, Kazuhiro; Kuniyoshi, Masami; Kobayashi, Kunihisa; Imamura, Minako; Inoguchi, Toyoshi; Nakamuta, Makoto; Nawata, Hajime.

:: In Vitro Cellular and Developmental Biology - Animal, 巻 41, 番号 10, 01.11.2005, p. 321-324.

研究成果: ジャーナルへの寄稿記事

Fukushima, M, Enjoji, M, Kohjima, M, Sugimoto, R, Ohta, S, Kotoh, K, Kuniyoshi, M, Kobayashi, K, Imamura, M, Inoguchi, T, Nakamuta, M & Nawata, H 2005, 'Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells', In Vitro Cellular and Developmental Biology - Animal, 巻. 41, 番号 10, pp. 321-324. https://doi.org/10.1290/0410069.1
Fukushima, Marie ; Enjoji, Munechika ; Kohjima, Motoyuki ; Sugimoto, Rie ; Ohta, Satoshi ; Kotoh, Kazuhiro ; Kuniyoshi, Masami ; Kobayashi, Kunihisa ; Imamura, Minako ; Inoguchi, Toyoshi ; Nakamuta, Makoto ; Nawata, Hajime. / Adipose differentiation related protein induces lipid accumulation and lipid droplet formation in hepatic stellate cells. :: In Vitro Cellular and Developmental Biology - Animal. 2005 ; 巻 41, 番号 10. pp. 321-324.
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