Adipose Tissue-Derived Stem Cell Imaging Using Cadmium-Free Quantum Dots

Yoshiyuki Miyazaki, Hiroshi Yukawa, Hiroyasu Nishi, Yukihiro Okamoto, Noritada Kaji, Tsukasa Torimoto, Yoshinobu Baba

研究成果: Contribution to journalArticle査読

抄録

Quantum dots (QDs) have received much attention for biomolecule and cell imaging applications because of their superior optical properties such as high quantum efficiency, size-tunable emission, and resistance to photobleaching process. However, QDs that are commercially available contain cadmium (Cd), a highly toxic element. Thus, the development of Cd-free and less toxic QDs is strongly desired. In this study, we developed Cd-free QDs (ZnS-coated ZnS-AgInS2 solid solution nanoparticles with a sulfo group: ZnS-ZAIS-SO3H) and investigated the ability of this material to label stem cells. ZnS-ZAIS-SO3H could be transduced into mouse adipose tissue-derived stem cells (mASCs) using octaarginine peptides (R8), known as cell-penetrating peptides. The optimal ratio of ZnS-ZAIS-SO3H:R8 was found to be 1:100 for labeling mASCs. More than 80% of mASCs labeled with 500 nM ZnS-ZAIS-SO3H were found to be alive, and the proliferation rates of labeled mASCs were maintained at the same rate as that of nonlabeled mASCs. In addition, no abnormalities in the morphology of mASCs labeled with ZnS-ZAIS-SO3H could be observed. These data suggest that ZnS-ZAIS-SO3H may be effective for the labeling of mASCs.

本文言語英語
ページ(範囲)91-7
ページ数7
ジャーナルCell medicine
6
1-2
DOI
出版ステータス出版済み - 12 30 2013

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