Adriamycin combined with hyperthermia and dipyridamole is cytotoxic both in vitro and in vivo

Y. Sakaguchi, Y. Maehara, Y. Emi, S. Kohnoe, K. Sugimachi

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

The cytotoxicity of adriamyin (ADM) combined with hyperthermia (Hyp) and/or dipyridamole (DP) was investigated using B16 melanoma cells in vitro and in vivo. When ADM was combined with Hyp at 43 °C or DP in a dose of 5 u.V/ or with both, drug cytotoxicity enhanced the inhibition of colony formation and cell growth, and the effect was maximal when Hyp and DP were combined. Incubation with DP alone for 24 h. following ADM exposure, enhanced the toxicity even further (p < 0.01). Measurement of intracellular levels of ADM in the B16 melanoma cells showed that Hyp and DP increased accumulation of ADM, and that DP, but not Hyp. significantly suppressed the ADM excretion (p < 0.05). Growth of B16 melanoma implanted into subcutaneous tissue of the foot of C57BL mice was inhibited to a greater extent by the combination treatment of ADM and DP, ADM and Hyp, and also that of ADM, Hyp and DP, compared to the findings in cases of ADM or Hyp alone. As this trimodality treatment is effective both in vitro and in vivo, further study on possible clinical advantage is warranted.

本文言語英語
ページ(範囲)249-256
ページ数8
ジャーナルEuropean Surgical Research
24
4
DOI
出版ステータス出版済み - 1992

All Science Journal Classification (ASJC) codes

  • 外科

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