Affinity purification of multifunctional polymer nanoparticles

Yu Hoshino, Walter W. Haberaecker, Takashi Kodama, Zhiyang Zeng, Yoshio Okahata, Kenneth J. Shea

研究成果: Contribution to journalArticle査読

68 被引用数 (Scopus)


We report that multifunctional polymer nanoparticles approximately the size of a large protein can be 'purified', on the basis of peptide affinity just as antibodies, using an affinity chromatography strategy. The selection process takes advantage of the thermoresponsiveness of the nanoparticles allowing 'catch and release' of the target peptide by adjusting the temperature. Purified particles show much stronger affinity (Kdapp ≈ nM) and a narrower affinity distribution than the average of particles before purification (Kdapp > μM) at room temperature but can release the peptide just by changing the temperature. We anticipate this affinity selection will be general and become an integral step for the preparation of "plastic antibodies" with near-homogeneous and tailored affinity for target biomacromolecules.

ジャーナルJournal of the American Chemical Society
出版ステータス出版済み - 10 6 2010

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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