Affinity separation of messenger RNA by thermo-responsive polymer carrying oligo(dT).

T. Mori, F. Oda, D. Umeno, M. Murata, M. Maeda

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The conjugate between oligo(dT)16 and thermo-responsive polymer, poly(N-isopropylacrylamide), was prepared for isolation of poly(A)+ RNA from total RNA. The hybridization reaction between the conjugate and poly(A) (average length: 320 base) was equilibrated in 10 min, and all the poly(A) (16 nmol base for 24 nmol base of conjugate) was precipitated when raising the solution temperature to 35 degrees C. The precipitate was dissolved in water, and poly(A) was dissociated from the conjugate by heating to 65 degrees C. This separation system was successfully applied to the isolation of poly(A)+ RNA from total RNA.

本文言語英語
ページ(範囲)55-56
ページ数2
ジャーナルNucleic acids symposium series
42
DOI
出版ステータス出版済み - 1999

All Science Journal Classification (ASJC) codes

  • Medicine(all)

フィンガープリント 「Affinity separation of messenger RNA by thermo-responsive polymer carrying oligo(dT).」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル