Age-related accumulation of Ig VH gene somatic mutations in peripheral B cells from aged humans

Y. Chong, Hideyuki Ikematsu, K. Yamaji, M. Nishimura, S. Kashiwagi, J. Hayashi

研究成果: Contribution to journalArticle査読

29 被引用数 (Scopus)

抄録

To investigate age-related alterations in human humoral immunity, we analysed Ig heavy chain variable region genes expressed by peripheral B cells from young and aged individuals. Three hundred and twenty-seven cDNA sequences, 163 μ and 164 γ transcripts with VH5 family genes, were analysed for somatic hypermutation and VHDJH recombinational features. Unmutated and mutated μ transcripts were interpreted as being from naive and memory IgM B cells, respectively. In young and aged individuals, the percentages of naive IgM among total μ transcripts were 39% and 42%, respectively. D and JH segment usage in naive IgM from aged individuals was similar to that from young individuals. The mutational frequencies of memory IgM were similar in young and aged individuals. γtranscripts, which are regarded as being from memory IgG B cells, showed a significantly higher mutational frequency (7.6%) in aged than in young individuals (5.8%) (P < 0.01). These findings suggest that VHDJH recombinational diversity was preserved, but that the accumulation of somatic mutations in the IgG VH region was increased in aged humans. The accumulation of somatic mutations in IgG B cells during ageing may imply that an age-related alteration exists in the selection and/or maintenance of peripheral memory B cells.

本文言語英語
ページ(範囲)59-66
ページ数8
ジャーナルClinical and Experimental Immunology
133
1
DOI
出版ステータス出版済み - 7 1 2003

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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