TY - JOUR
T1 - Aggregation of cationic amphiphilic block and random copoly(vinyl ether)s with antimicrobial activity
AU - Oda, Yukari
AU - Yasuhara, Kazuma
AU - Kanaoka, Shokyoku
AU - Sato, Takahiro
AU - Aoshima, Sadahito
AU - Kuroda, Kenichi
N1 - Funding Information:
This research was partly supported by the Global Center of Excellence (G-COE) Program "Global Education and Research Center for Bio-Environmental Chemistry" at Osaka University (to Yukari Oda), NSF CAREER Award (DMR-0845592) (to Kenichi Kuroda), and Department of Biologic and Materials Sciences, University of Michigan School of Dentistry. We thank Takeshi Suwabe at School of Dentistry, University of Michigan, for his help on the fluorescence microscopic observations.
Publisher Copyright:
© 2018 by the authors.
PY - 2018/1/19
Y1 - 2018/1/19
N2 - In this study, we investigated the aggregation behaviors of amphiphilic poly(vinyl ether)s with antimicrobial activity. We synthesized a di-block poly(vinyl ether), B3826, composed of cationic primary amine and hydrophobic isobutyl (iBu) side chains, which previously showed antimicrobial activity against Escherichia coli. B3826 showed similar uptake behaviors as those for a hydrophobic fluorescent dye, 1,6-diphenyl-1,3,5-hexatriene, to counterpart polymers including homopolymer H44 and random copolymer R4025, indicating that the iBu block does not form strong hydrophobic domains. The cryo-TEM observations also indicated that the polymer aggregate of B3826 appears to have low-density polymer chains without any defined microscopic structures. We speculate that B3826 formed large aggregates by liquid-liquid separation due to the weak association of polymer chains. The fluorescence microscopy images showed that B3826 bonds to E. coli cell surfaces, and these bacterial cells were stained by propidium iodide, indicating that the cell membranes were significantly damaged. The results suggest that block copolymers may provide a new platform to design and develop antimicrobial materials that can utilize assembled structures and properties.
AB - In this study, we investigated the aggregation behaviors of amphiphilic poly(vinyl ether)s with antimicrobial activity. We synthesized a di-block poly(vinyl ether), B3826, composed of cationic primary amine and hydrophobic isobutyl (iBu) side chains, which previously showed antimicrobial activity against Escherichia coli. B3826 showed similar uptake behaviors as those for a hydrophobic fluorescent dye, 1,6-diphenyl-1,3,5-hexatriene, to counterpart polymers including homopolymer H44 and random copolymer R4025, indicating that the iBu block does not form strong hydrophobic domains. The cryo-TEM observations also indicated that the polymer aggregate of B3826 appears to have low-density polymer chains without any defined microscopic structures. We speculate that B3826 formed large aggregates by liquid-liquid separation due to the weak association of polymer chains. The fluorescence microscopy images showed that B3826 bonds to E. coli cell surfaces, and these bacterial cells were stained by propidium iodide, indicating that the cell membranes were significantly damaged. The results suggest that block copolymers may provide a new platform to design and develop antimicrobial materials that can utilize assembled structures and properties.
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U2 - 10.3390/polym10010093
DO - 10.3390/polym10010093
M3 - Article
AN - SCOPUS:85040769348
VL - 10
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 1
M1 - 93
ER -