The preparation of poly(amido amine) (PAMAM) dendrimer monolayers at the air/water interface was analyzed. Investigations show the transfer of PAMAM dedrimer monolayers from the air side onto hydrophobic silicon wafers, which were treated by the horizontal lifting method. X-ray reflectivity, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) techniques were used to study the aggregation states in the PAMAM monolayers. The results show that the PAMAM molecules sit on the substrate with an oblate shape in which the hydrophilic core and hydrophobic alkyl end groups were towards the substrate and air sides.
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