Agrobacterium-mediated transformation of japonica rice using mature embryos and regenerated transgenic plants

Ammar Elakhdar, Masako Fukuda, Takahiko Kubo

研究成果: Contribution to journalArticle査読

抄録

Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica and indica varieties. In this protocol, we elucidate a rapid and highly efficient protocol to transform and regenerate transgenic rice plants through important key features of Agrobacterium transformation and standard regeneration media, especially enhancing culture conditions, timing, and growth hormones. With this protocol, transformed plantlets from the embryogenetic callus of the japonica cultivar 'Taichung 65' may be obtained within 90 days. This protocol may be used with other japonica rice varieties.

本文言語英語
論文番号e4143
ジャーナルBio-protocol
11
18
DOI
出版ステータス出版済み - 9 20 2021

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)
  • 神経科学(全般)
  • 植物科学

フィンガープリント

「Agrobacterium-mediated transformation of japonica rice using mature embryos and regenerated transgenic plants」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル