Akt plays a central role in the anti-apoptotic effect of estrogen in endothelial cells

Miho Koga, Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Hitoo Nakano, Hideo Kanaide

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Estrogen has been reported to inhibit apoptosis in vascular endothelial cells. However, its precise mechanism still remains to be elucidated. Here we determined the role of Akt in the anti-apoptotic effect of estrogen. 17β-Estradiol prevented the apoptosis induced by TNF-α in bovine aortic endothelial cells, as evaluated by double staining with fluorescein isothiocyanate-conjugated annexin V and propidium iodide. Introducing a dominant negative mutant of Akt by using a cell-penetrating peptide of Tat protein inhibited the anti-apoptotic effect of estrogen in a concentration-dependent manner, and resulted in the complete inhibition of the anti-apoptotic effect of 17β-estradiol at 1 nM and higher concentrations. The dominant negative mutant without the cell-penetrating peptide and Tat peptide-conjugated protein A had no effect. The intracellular protein transduction was confirmed by immunoblot analysis. Our observations thus provide first direct evidence that Akt plays a central role in the anti-apoptotic effect of estrogen in vascular endothelial cells.

元の言語英語
ページ(範囲)321-325
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
324
発行部数1
DOI
出版物ステータス出版済み - 11 5 2004

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Endothelial cells
Estrogens
Endothelial Cells
Cell-Penetrating Peptides
Estradiol
tat Gene Products
Apoptosis
Propidium
Annexin A5
Staphylococcal Protein A
Fluorescein
Staining and Labeling
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Akt plays a central role in the anti-apoptotic effect of estrogen in endothelial cells. / Koga, Miho; Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Nakano, Hitoo; Kanaide, Hideo.

:: Biochemical and Biophysical Research Communications, 巻 324, 番号 1, 05.11.2004, p. 321-325.

研究成果: ジャーナルへの寄稿記事

Koga, Miho ; Hirano, Katsuya ; Hirano, Mayumi ; Nishimura, Junji ; Nakano, Hitoo ; Kanaide, Hideo. / Akt plays a central role in the anti-apoptotic effect of estrogen in endothelial cells. :: Biochemical and Biophysical Research Communications. 2004 ; 巻 324, 番号 1. pp. 321-325.
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