TY - JOUR
T1 - Albumin permeability across endothelial monolayers under long-term shear stress
AU - Kudo, Susumu
AU - Tsuzaka, Masanori
AU - Ikeda, Mariko
AU - Tanishita, Kazuo
PY - 2006/6/15
Y1 - 2006/6/15
N2 - Endothelial cells lining the inner surface of blood vessels regulate the exchange of molecules between the blood and the vessel wall. This study investigates the effect of longterm shear stress on macromolecule permeability across endothelial monolayers. In vitro system to measure transendothelial permeability to tetramethylrhodamine conjugated albumin under shear stress was developed. The temporal variation in albumin permeability under 1 Pa and 4 Pa over 48 h was quantified using a fluorescence spectrophotometer. At 4 Pa, albumin permeability did not show a statistical significant change over 48 h. Under shear stress of 1 Pa, the average albumin permeability between 12 and 30 h was 2.5-fold higher than the average between 0 and 6 h. Under shear stress of 1 Pa, the average albumin permeability between 36 and 48 h was 1.8-fold higher than the average between 12 and 30 h. These results show that both the magnitude of, and the length of exposure to, shear stress regulate transendothelial permeability.
AB - Endothelial cells lining the inner surface of blood vessels regulate the exchange of molecules between the blood and the vessel wall. This study investigates the effect of longterm shear stress on macromolecule permeability across endothelial monolayers. In vitro system to measure transendothelial permeability to tetramethylrhodamine conjugated albumin under shear stress was developed. The temporal variation in albumin permeability under 1 Pa and 4 Pa over 48 h was quantified using a fluorescence spectrophotometer. At 4 Pa, albumin permeability did not show a statistical significant change over 48 h. Under shear stress of 1 Pa, the average albumin permeability between 12 and 30 h was 2.5-fold higher than the average between 0 and 6 h. Under shear stress of 1 Pa, the average albumin permeability between 36 and 48 h was 1.8-fold higher than the average between 12 and 30 h. These results show that both the magnitude of, and the length of exposure to, shear stress regulate transendothelial permeability.
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U2 - 10.1299/jsmec.48.419
DO - 10.1299/jsmec.48.419
M3 - Article
AN - SCOPUS:33745198434
VL - 48
SP - 419
EP - 424
JO - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
JF - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
SN - 1344-7653
IS - 4
ER -