Alcohol-tolerant mutants of cyanobacterium Synechococcus elongatus PCC 7942 obtained by single-cell mutant screening system

Sayuri Arai, Kayoko Hayashihara, Yuki Kanamoto, Kazunori Shimizu, Yasutaka Hirokawa, Taizo Hanai, Akio Murakami, Hiroyuki Honda

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Enhancement of alcohol tolerance in microorganisms is an important strategy for improving bioalcohol productivity. Although cyanobacteria can be used as a promising biocatalyst to produce various alcohols directly from CO2, low productivity, and low tolerance against alcohols are the main issues to be resolved. Nevertheless, to date, a mutant with increasing alcohol tolerance has rarely been reported. In this study, we attempted to select isopropanol (IPA)-tolerant mutants of Synechococcus elongatus PCC 7942 using UV-C-induced random mutagenesis, followed by enrichment of the tolerant candidates in medium containing 10 g/L IPA and screening of the cells with a high growth rate in the single cell culture system in liquid medium containing 10 g/L IPA. We successfully acquired the most tolerant strain, SY1043, which maintains the ability to grow in medium containing 30 g/L IPA. The photosynthetic oxygen-evolving activities of SY1043 were almost same in cells after 72 h incubation under light with or without 10 g/L IPA, while the activity of the wild-type was remarkably decreased after the incubation with IPA. SY1043 also showed higher tolerance to ethanol, 1-butanol, isobutanol, and 1-pentanol than the wild type. These results suggest that SY1043 would be a promising candidate to improve alcohol production using cyanobacteria. Biotechnol. Bioeng. 2017;114: 1771–1778.

元の言語英語
ページ(範囲)1771-1778
ページ数8
ジャーナルBiotechnology and Bioengineering
114
発行部数8
DOI
出版物ステータス出版済み - 8 1 2017

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Synechococcus
2-Propanol
Cyanobacteria
Screening
Alcohols
Productivity
Mutagenesis
Biocatalysts
1-Butanol
Butenes
Cell culture
Microorganisms
Ethanol
Cell Culture Techniques
Oxygen
Light
Liquids
Enzymes
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Alcohol-tolerant mutants of cyanobacterium Synechococcus elongatus PCC 7942 obtained by single-cell mutant screening system. / Arai, Sayuri; Hayashihara, Kayoko; Kanamoto, Yuki; Shimizu, Kazunori; Hirokawa, Yasutaka; Hanai, Taizo; Murakami, Akio; Honda, Hiroyuki.

:: Biotechnology and Bioengineering, 巻 114, 番号 8, 01.08.2017, p. 1771-1778.

研究成果: ジャーナルへの寄稿記事

Arai, S, Hayashihara, K, Kanamoto, Y, Shimizu, K, Hirokawa, Y, Hanai, T, Murakami, A & Honda, H 2017, 'Alcohol-tolerant mutants of cyanobacterium Synechococcus elongatus PCC 7942 obtained by single-cell mutant screening system', Biotechnology and Bioengineering, 巻. 114, 番号 8, pp. 1771-1778. https://doi.org/10.1002/bit.26307
Arai, Sayuri ; Hayashihara, Kayoko ; Kanamoto, Yuki ; Shimizu, Kazunori ; Hirokawa, Yasutaka ; Hanai, Taizo ; Murakami, Akio ; Honda, Hiroyuki. / Alcohol-tolerant mutants of cyanobacterium Synechococcus elongatus PCC 7942 obtained by single-cell mutant screening system. :: Biotechnology and Bioengineering. 2017 ; 巻 114, 番号 8. pp. 1771-1778.
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