Alginate/aminopropyl-silicate/alginate membrane immunoisolatability and insulin secretion of encapsulated islets

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

研究成果: Contribution to journalArticle

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We utilized the sol-gel reaction to prepare an immunoisolatable membrane for a microcapsule-shaped bioartificial pancreas. The membrane, derived from two precursors, 3-(aminopropyl)trimethoxysilane (APTrMOS) and tetramethoxysilane (TMOS), was formed onto calcium-alginate gel beads via electrostatic interaction. The molecular weight cutoff point of less than 150 000 required for immunoisolation was achieved at molar ratios ([APTrMOS]/[TMOS]) ranging from 0.60 to 2.40 with the amount of APTrMOS fixed at 3.40 mmol/(10 mL of calcium-alginate). When encapsulated in a membrane prepared at the molar ratio of 0.60, the islets contracted in volume and showed no response to stimulation by a high glucose concentration. However, islets in a membrane prepared at the molar ratio of 2.40 showed no contraction and responded to the glucose stimulation at almost the same level as free islets. These results demonstrated that the molar ratio of the precursors was a dominant factor affecting membrane permeability and the insulin secretion activity of the encapsulated islets.

元の言語英語
ページ(範囲)401-403
ページ数3
ジャーナルBiotechnology Progress
18
発行部数2
DOI
出版物ステータス出版済み - 4 22 2002

All Science Journal Classification (ASJC) codes

  • Biotechnology

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