Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro

Akira Iwamoto, Yuichi Inoue, Hirofumi Tachibana, Hiroharu Kawahara

研究成果: ジャーナルへの寄稿記事

抄録

We found that strawberry (Fragaria x ananassa) extract has an IgE production suppressive activity and its oral administration improved skin manifestation in atopic dermatitis model mice. In present study, we identified an active substance using the IgE-producing human myeloma cell line U266. Gel filtration experiment indicated that the IgE suppressor was more than 6 kDa in molecular size. In addition, its pectinase treatment inhibited the activity, suggesting that the active substance in strawberry extract is pectin. Among solutions of water-(WP), hexametaphosphate-(HXP), acid-(HP) and alkali soluble pectin (OHP) extracted from strawberry, only OHP suppressed IgE production, and their suppressive activity was cancelled by pectinase treatment. In addition, OHP extracted from apple also inhibited IgE production. Furthermore, OHP also suppressed IgE production and did not affect IgG and IgM production in human peripheral blood mononuclear cells in an in vitro immunization condition. From these results, we concluded that OHP was an IgE suppressor in strawberry extract.

元の言語英語
ページ(範囲)573-581
ページ数9
ジャーナルCytotechnology
71
発行部数2
DOI
出版物ステータス出版済み - 4 15 2019

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Alkalies
Immunoglobulin E
Fragaria
Cells
Cell Line
Polygalacturonase
Immunization
Skin Manifestations
Skin
Blood
Gels
Malus
Atopic Dermatitis
pectin
In Vitro Techniques
Gel Chromatography
Immunoglobulin M
Oral Administration
Blood Cells
Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

これを引用

Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro. / Iwamoto, Akira; Inoue, Yuichi; Tachibana, Hirofumi; Kawahara, Hiroharu.

:: Cytotechnology, 巻 71, 番号 2, 15.04.2019, p. 573-581.

研究成果: ジャーナルへの寄稿記事

Iwamoto, Akira ; Inoue, Yuichi ; Tachibana, Hirofumi ; Kawahara, Hiroharu. / Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro. :: Cytotechnology. 2019 ; 巻 71, 番号 2. pp. 573-581.
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