Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry

Takanobu Nobori, Kenta Tosaka, Akira Kawamura, Taisei Joichi, Kenta Kamino, Akihiro Kishimura, Eishi Baba, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Despite the expanding use of flow cytometry, its detection limit is not satisfactory for many antigen proteins with low copy numbers. Herein, we describe an alkaline phosphatase (AP)-based technique to amplify the fluorescence signal for cell staining applications. We designed a fluorescent substrate that acquires membrane permeability upon dephosphorylation by AP. By using the substrate, the fluorescence signal of cells in flow cytometry could be successfully amplified to give a much stronger signal than the cells labeled using a conventional fluorophore-modified antibody.

元の言語英語
ページ(範囲)1059-1062
ページ数4
ジャーナルAnalytical Chemistry
90
発行部数2
DOI
出版物ステータス出版済み - 1 16 2018

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Flow cytometry
Amplification
Alkaline Phosphatase
Fluorescence
Fluorophores
Substrates
Membranes
Antigens
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry. / Nobori, Takanobu; Tosaka, Kenta; Kawamura, Akira; Joichi, Taisei; Kamino, Kenta; Kishimura, Akihiro; Baba, Eishi; Mori, Takeshi; Katayama, Yoshiki.

:: Analytical Chemistry, 巻 90, 番号 2, 16.01.2018, p. 1059-1062.

研究成果: ジャーナルへの寄稿記事

Nobori, Takanobu ; Tosaka, Kenta ; Kawamura, Akira ; Joichi, Taisei ; Kamino, Kenta ; Kishimura, Akihiro ; Baba, Eishi ; Mori, Takeshi ; Katayama, Yoshiki. / Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry. :: Analytical Chemistry. 2018 ; 巻 90, 番号 2. pp. 1059-1062.
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AU - Kishimura, Akihiro

AU - Baba, Eishi

AU - Mori, Takeshi

AU - Katayama, Yoshiki

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