Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry

Takanobu Nobori, Kenta Tosaka, Akira Kawamura, Taisei Joichi, Kenta Kamino, Akihiro Kishimura, Eishi Baba, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿学術誌査読

11 被引用数 (Scopus)

抄録

Despite the expanding use of flow cytometry, its detection limit is not satisfactory for many antigen proteins with low copy numbers. Herein, we describe an alkaline phosphatase (AP)-based technique to amplify the fluorescence signal for cell staining applications. We designed a fluorescent substrate that acquires membrane permeability upon dephosphorylation by AP. By using the substrate, the fluorescence signal of cells in flow cytometry could be successfully amplified to give a much stronger signal than the cells labeled using a conventional fluorophore-modified antibody.

本文言語英語
ページ(範囲)1059-1062
ページ数4
ジャーナルAnalytical Chemistry
90
2
DOI
出版ステータス出版済み - 1月 16 2018

!!!All Science Journal Classification (ASJC) codes

  • 分析化学

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