Alterations in B-cell subsets in pediatric patients with early atopic dermatitis

Tali Czarnowicki, Hitokazu Esaki, Juana Gonzalez, Yael Renert-Yuval, Patrick Brunner, Margeaux Oliva, Yeriel Estrada, Hui Xu, Xiuzhong Zheng, Sreya Talasila, Isabel Haugh, Thy Huynh, Sarah Lyon, Gary Tran, Hugh Sampson, Mayte Suárez-Fariñas, James G. Krueger, Emma Guttman-Yassky, Amy S. Paller

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Background B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD). Objective We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching. Methods We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP. Results Compared with adults, children showed T-cell predominance in the skin. Circulating CD19+CD20+ B-cell counts were lower in patients with pediatric AD than in control subjects (24% vs 33%, P =.04), whereas CD3+ T-cell counts were higher (62% vs 52%, P =.05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects (r = −0.48, P =.07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts (r = 0.42, P =.03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD (P <.0001). Diverse sIgE levels correlated with SCORAD scores and age of pediatric patients with AD (P <.01). Positive correlations were observed between activated B-cell and memory T-cell counts (P <.02). In patients with AD, IgE sensitization to most allergens clustered with age, TH1, TH2, total IgE levels, and B-cell memory subsets. Conclusions Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions.

元の言語英語
ページ(範囲)134-144.e9
ジャーナルJournal of Allergy and Clinical Immunology
140
発行部数1
DOI
出版物ステータス出版済み - 7 2017

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Czarnowicki, T., Esaki, H., Gonzalez, J., Renert-Yuval, Y., Brunner, P., Oliva, M., Estrada, Y., Xu, H., Zheng, X., Talasila, S., Haugh, I., Huynh, T., Lyon, S., Tran, G., Sampson, H., Suárez-Fariñas, M., Krueger, J. G., Guttman-Yassky, E., & Paller, A. S. (2017). Alterations in B-cell subsets in pediatric patients with early atopic dermatitis. Journal of Allergy and Clinical Immunology, 140(1), 134-144.e9. https://doi.org/10.1016/j.jaci.2016.09.060