Alterations in the Duodenal Fluid Microbiome of Patients With Pancreatic Cancer

Shiro Kohi, Anne Macgregor-Das, Mohamad Dbouk, Takeichi Yoshida, Miguel Chuidian, Toshiya Abe, Michael Borges, Anne Marie Lennon, Eun Ji Shin, Marcia Irene Canto, Michael Goggins

研究成果: ジャーナルへの寄稿学術誌査読

14 被引用数 (Scopus)

抄録

Background & Aims: The tumor microbiome of patients with pancreas ductal adenocarcinoma (PDAC) includes bacteria normally present in the upper gastrointestinal tract. If the predominant source of intratumoral bacteria in patients with PDAC is retrograde migration from the duodenum, duodenal fluid could be a representative biospecimen for determining microbiome profiles of patients with PDAC or at risk of developing PDAC. Methods: We performed a case-control study comparing bacterial and fungal (16S and 18S rRNA) profiles of secretin-stimulated duodenal fluid collections from 308 patients undergoing duodenal endoscopy including 134 normal pancreas control subjects, 98 patients with pancreatic cyst(s) and 74 patients with PDAC. Results: Alterations in duodenal fluid microbiomes with diminished alpha diversity were significantly associated with age >70 and proton pump inhibitor use. Patients with PDAC had significantly decreased duodenal microbial alpha diversity compared with age-matched control subjects with normal pancreata and those with pancreatic cyst(s). There was evidence of enrichment of Bifidobacterium genera in the duodenal fluid of patients with PDAC compared with control subjects and those with pancreatic cyst(s). There were also enrichment of duodenal fluid Fusobacteria and Rothia bacteria among patients with PDAC with short-term survival. Duodenal fluid microbiome profiles were not significantly different between control subjects and patients with pancreatic cyst(s). Conclusion: Patients with PDAC have alterations in their duodenal fluid microbiome profiles compared with patients with pancreatic cysts and those with normal pancreata. ClinicalTrials.gov,

本文言語英語
ページ(範囲)e196-e227
ジャーナルClinical Gastroenterology and Hepatology
20
2
DOI
出版ステータス出版済み - 2月 2022
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 肝臓学
  • 消化器病学

フィンガープリント

「Alterations in the Duodenal Fluid Microbiome of Patients With Pancreatic Cancer」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル