Alternative oxidase capacity of mitochondria in microsporophylls may function in cycad thermogenesis

Yasuko Ito Inaba, Mayuko Sato, Mitsuhiko P. Sato, Yuya Kurayama, Haruna Yamamoto, Mizuki Ohata, Yoshitoshi Ogura, Tetsuya Hayashi, Kiminori Toyooka, Takehito Inaba

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Cone thermogenesis is a widespread phenomenon in cycads and may function to promote volatile emissions that affect pollinator behavior. Given their large population size and intense and durable heat-producing effects, cycads are important organisms for comprehensive studies of plant thermogenesis. However, knowledge of mitochondrial morphology and function in cone thermogenesis is limited. Therefore, we investigated these mitochondrial properties in the thermogenic cycad species Cycas revoluta. Male cones generated heat even in cool weather conditions. Female cones produced heat, but to a lesser extent than male cones. Ultrastructural analyses of the two major tissues of male cones, microsporophylls and microsporangia, revealed the existence of a population of mitochondria with a distinct morphology in the microsporophylls. In these cells, we observed large mitochondria (cross-sectional area of 2 mm2 or more) with a uniform matrix density that occupied .10% of the total mitochondrial volume. Despite the size difference, many nonlarge mitochondria (cross-sectional area ,2 mm2) also exhibited a shape and a matrix density similar to those of large mitochondria. Alternative oxidase (AOX) capacity and expression levels in microsporophylls were much higher than those in microsporangia. The AOX genes expressed in male cones revealed two different AOX complementary DNA sequences: CrAOX1 and CrAOX2. The expression level of CrAOX1 mRNA in the microsporophylls was 100 times greater than that of CrAOX2 mRNA. Collectively, these results suggest that distinctive mitochondrial morphology and CrAOX1-mediated respiration in microsporophylls might play a role in cycad cone thermogenesis.

元の言語英語
ページ(範囲)743-756
ページ数14
ジャーナルPlant physiology
180
発行部数2
DOI
出版物ステータス出版済み - 6 2019

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Cycadopsida
seed cones
Thermogenesis
heat production
Mitochondria
mitochondria
Hot Temperature
Cycas
Mitochondrial Size
Messenger RNA
Weather
Population Density
heat
Respiration
Cycas revoluta
Complementary DNA
complementary DNA
alternative oxidase
cell respiration
pollinators

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

これを引用

Inaba, Y. I., Sato, M., Sato, M. P., Kurayama, Y., Yamamoto, H., Ohata, M., ... Inaba, T. (2019). Alternative oxidase capacity of mitochondria in microsporophylls may function in cycad thermogenesis. Plant physiology, 180(2), 743-756. https://doi.org/10.1104/pp.19.00150

Alternative oxidase capacity of mitochondria in microsporophylls may function in cycad thermogenesis. / Inaba, Yasuko Ito; Sato, Mayuko; Sato, Mitsuhiko P.; Kurayama, Yuya; Yamamoto, Haruna; Ohata, Mizuki; Ogura, Yoshitoshi; Hayashi, Tetsuya; Toyooka, Kiminori; Inaba, Takehito.

:: Plant physiology, 巻 180, 番号 2, 06.2019, p. 743-756.

研究成果: ジャーナルへの寄稿記事

Inaba, YI, Sato, M, Sato, MP, Kurayama, Y, Yamamoto, H, Ohata, M, Ogura, Y, Hayashi, T, Toyooka, K & Inaba, T 2019, 'Alternative oxidase capacity of mitochondria in microsporophylls may function in cycad thermogenesis', Plant physiology, 巻. 180, 番号 2, pp. 743-756. https://doi.org/10.1104/pp.19.00150
Inaba, Yasuko Ito ; Sato, Mayuko ; Sato, Mitsuhiko P. ; Kurayama, Yuya ; Yamamoto, Haruna ; Ohata, Mizuki ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Toyooka, Kiminori ; Inaba, Takehito. / Alternative oxidase capacity of mitochondria in microsporophylls may function in cycad thermogenesis. :: Plant physiology. 2019 ; 巻 180, 番号 2. pp. 743-756.
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abstract = "Cone thermogenesis is a widespread phenomenon in cycads and may function to promote volatile emissions that affect pollinator behavior. Given their large population size and intense and durable heat-producing effects, cycads are important organisms for comprehensive studies of plant thermogenesis. However, knowledge of mitochondrial morphology and function in cone thermogenesis is limited. Therefore, we investigated these mitochondrial properties in the thermogenic cycad species Cycas revoluta. Male cones generated heat even in cool weather conditions. Female cones produced heat, but to a lesser extent than male cones. Ultrastructural analyses of the two major tissues of male cones, microsporophylls and microsporangia, revealed the existence of a population of mitochondria with a distinct morphology in the microsporophylls. In these cells, we observed large mitochondria (cross-sectional area of 2 mm2 or more) with a uniform matrix density that occupied .10{\%} of the total mitochondrial volume. Despite the size difference, many nonlarge mitochondria (cross-sectional area ,2 mm2) also exhibited a shape and a matrix density similar to those of large mitochondria. Alternative oxidase (AOX) capacity and expression levels in microsporophylls were much higher than those in microsporangia. The AOX genes expressed in male cones revealed two different AOX complementary DNA sequences: CrAOX1 and CrAOX2. The expression level of CrAOX1 mRNA in the microsporophylls was 100 times greater than that of CrAOX2 mRNA. Collectively, these results suggest that distinctive mitochondrial morphology and CrAOX1-mediated respiration in microsporophylls might play a role in cycad cone thermogenesis.",
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AU - Sato, Mayuko

AU - Sato, Mitsuhiko P.

AU - Kurayama, Yuya

AU - Yamamoto, Haruna

AU - Ohata, Mizuki

AU - Ogura, Yoshitoshi

AU - Hayashi, Tetsuya

AU - Toyooka, Kiminori

AU - Inaba, Takehito

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