Alternative Pathway-Hemolytic Complement Titer (ACH50) of Porgy Pagrus major Serum

Tomoki Yano, Hiroko Matsuyama, Miki Nakao, Koji Tanaka

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

Alternative complement pathway activity (ACH50) of porgy serum was measured by incubating the serum with rabbit red blood cells (RaRBC) in the presence of 10 Mm Egta. It was confirmed that the concentration of EGTA (10 Mm) Is enough to prevent the activation of the classical pathway of porgy complement, and that among red blood cells from several animals, RaRBC is the most effective activator of the alternative pathway of porgy complement. Optimum conditions for the hemolytic reaction were: reaction temperature, 15°C; reaction time, 120 min; pH 7.8; concentration of Mg2+, 10 mM. When determined under these conditions, the ACH 50 values of the sera from 33 porgy, weighing 23–31 g, were remarkably high (143 ± 81 units/ml) as compared with those reported for mammalian sera.

本文言語英語
ページ(範囲)1887-1891
ページ数5
ジャーナルNIPPON SUISAN GAKKAISHI
53
10
DOI
出版ステータス出版済み - 1987

All Science Journal Classification (ASJC) codes

  • 水圏科学

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