Amelogenin is a negative regulator of osteoclastogenesis via downregulation of RANKL, M-CSF and fibronectin expression in osteoblasts

Miyuki Nishiguchi, Kenji Yuasa, Kan Saito, Emiko Fukumoto, Aya Yamada, Tomokazu Hasegawa, Keigo Yoshizaki, Yoko Kamasaki, Kazuaki Nonaka, Taku Fujiwara, Satoshi Fukumoto

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

Amelogenin is a novel enamel matrix protein. Knockout mice showed enhanced osteoclast formation and resorption of tooth cementum. This study investigated the effects of amelogenin on osteoclastogenesis. In co-cultures with calvaria osteoblasts and purified bone marrow cells, amelogenin inhibited osteoclastogenesis dramatically. Furthermore, amelogenin inhibited the expression of receptor activator of nuclear factor κB ligand (RANKL), macrophage-colony stimulating factor (M-CSF) and fibronectin in osteoblasts, while RANKL expression was induced by fibronectin and inhibited by treatment with fibronectin small interfering RNA. These results suggest that the inhibitory effects of amelogenin on osteoclastogenesis lead to downregulation of RANKL, M-CSF and fibronectin production in osteoblasts.

元の言語英語
ページ(範囲)237-243
ページ数7
ジャーナルArchives of Oral Biology
52
発行部数3
DOI
出版物ステータス出版済み - 3 1 2007

Fingerprint

Amelogenin
Macrophage Colony-Stimulating Factor
Osteoblasts
Fibronectins
Osteogenesis
Down-Regulation
Tooth Resorption
Dental Cementum
Osteoclasts
Cytoplasmic and Nuclear Receptors
Coculture Techniques
Skull
Knockout Mice
Bone Marrow Cells
Small Interfering RNA
Ligands

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

これを引用

Amelogenin is a negative regulator of osteoclastogenesis via downregulation of RANKL, M-CSF and fibronectin expression in osteoblasts. / Nishiguchi, Miyuki; Yuasa, Kenji; Saito, Kan; Fukumoto, Emiko; Yamada, Aya; Hasegawa, Tomokazu; Yoshizaki, Keigo; Kamasaki, Yoko; Nonaka, Kazuaki; Fujiwara, Taku; Fukumoto, Satoshi.

:: Archives of Oral Biology, 巻 52, 番号 3, 01.03.2007, p. 237-243.

研究成果: ジャーナルへの寄稿記事

Nishiguchi, Miyuki ; Yuasa, Kenji ; Saito, Kan ; Fukumoto, Emiko ; Yamada, Aya ; Hasegawa, Tomokazu ; Yoshizaki, Keigo ; Kamasaki, Yoko ; Nonaka, Kazuaki ; Fujiwara, Taku ; Fukumoto, Satoshi. / Amelogenin is a negative regulator of osteoclastogenesis via downregulation of RANKL, M-CSF and fibronectin expression in osteoblasts. :: Archives of Oral Biology. 2007 ; 巻 52, 番号 3. pp. 237-243.
@article{107a2d40b25b4206916bf16d89d7e47a,
title = "Amelogenin is a negative regulator of osteoclastogenesis via downregulation of RANKL, M-CSF and fibronectin expression in osteoblasts",
abstract = "Amelogenin is a novel enamel matrix protein. Knockout mice showed enhanced osteoclast formation and resorption of tooth cementum. This study investigated the effects of amelogenin on osteoclastogenesis. In co-cultures with calvaria osteoblasts and purified bone marrow cells, amelogenin inhibited osteoclastogenesis dramatically. Furthermore, amelogenin inhibited the expression of receptor activator of nuclear factor κB ligand (RANKL), macrophage-colony stimulating factor (M-CSF) and fibronectin in osteoblasts, while RANKL expression was induced by fibronectin and inhibited by treatment with fibronectin small interfering RNA. These results suggest that the inhibitory effects of amelogenin on osteoclastogenesis lead to downregulation of RANKL, M-CSF and fibronectin production in osteoblasts.",
author = "Miyuki Nishiguchi and Kenji Yuasa and Kan Saito and Emiko Fukumoto and Aya Yamada and Tomokazu Hasegawa and Keigo Yoshizaki and Yoko Kamasaki and Kazuaki Nonaka and Taku Fujiwara and Satoshi Fukumoto",
year = "2007",
month = "3",
day = "1",
doi = "10.1016/j.archoralbio.2006.09.016",
language = "English",
volume = "52",
pages = "237--243",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Amelogenin is a negative regulator of osteoclastogenesis via downregulation of RANKL, M-CSF and fibronectin expression in osteoblasts

AU - Nishiguchi, Miyuki

AU - Yuasa, Kenji

AU - Saito, Kan

AU - Fukumoto, Emiko

AU - Yamada, Aya

AU - Hasegawa, Tomokazu

AU - Yoshizaki, Keigo

AU - Kamasaki, Yoko

AU - Nonaka, Kazuaki

AU - Fujiwara, Taku

AU - Fukumoto, Satoshi

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Amelogenin is a novel enamel matrix protein. Knockout mice showed enhanced osteoclast formation and resorption of tooth cementum. This study investigated the effects of amelogenin on osteoclastogenesis. In co-cultures with calvaria osteoblasts and purified bone marrow cells, amelogenin inhibited osteoclastogenesis dramatically. Furthermore, amelogenin inhibited the expression of receptor activator of nuclear factor κB ligand (RANKL), macrophage-colony stimulating factor (M-CSF) and fibronectin in osteoblasts, while RANKL expression was induced by fibronectin and inhibited by treatment with fibronectin small interfering RNA. These results suggest that the inhibitory effects of amelogenin on osteoclastogenesis lead to downregulation of RANKL, M-CSF and fibronectin production in osteoblasts.

AB - Amelogenin is a novel enamel matrix protein. Knockout mice showed enhanced osteoclast formation and resorption of tooth cementum. This study investigated the effects of amelogenin on osteoclastogenesis. In co-cultures with calvaria osteoblasts and purified bone marrow cells, amelogenin inhibited osteoclastogenesis dramatically. Furthermore, amelogenin inhibited the expression of receptor activator of nuclear factor κB ligand (RANKL), macrophage-colony stimulating factor (M-CSF) and fibronectin in osteoblasts, while RANKL expression was induced by fibronectin and inhibited by treatment with fibronectin small interfering RNA. These results suggest that the inhibitory effects of amelogenin on osteoclastogenesis lead to downregulation of RANKL, M-CSF and fibronectin production in osteoblasts.

UR - http://www.scopus.com/inward/record.url?scp=33846226701&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846226701&partnerID=8YFLogxK

U2 - 10.1016/j.archoralbio.2006.09.016

DO - 10.1016/j.archoralbio.2006.09.016

M3 - Article

C2 - 17101114

AN - SCOPUS:33846226701

VL - 52

SP - 237

EP - 243

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 3

ER -