Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration

J. Hatakeyama, D. Philp, Y. Hatakeyama, N. Haruyama, L. Shum, M. A. Aragon, Z. Yuan, C. W. Gibson, T. Sreenath, H. K. Kleinman, A. B. Kulkami

研究成果: Contribution to journalArticle査読

54 被引用数 (Scopus)

抄録

We previously reported that amelogenin isoforms M180 and leucine-rich amelogenin peptide (LRAP) are expressed in the periodontal region, and that their absence is associated with increased cementum defects in amelogenin-knockout (KO) mice. The aim of the present study was to characterize the functions of these isoforms in osteoclastogenesis and in the proliferation and migration of cementoblast/periodontal ligament cells. The co-cultures of wild-type (WT) osteoclast progenitor and KO cementoblast/periodontal ligament cells displayed more tartrate-resistant acid phosphatase (TRAP)-positive cells than the co-cultures of WT cells. The addition of LRAP to both co-cultures significantly reduced RANKL expression and the TRAP-positive cells. Proliferation and migration rates of the KO cementoblast/periodontal ligament cells were lower than those of WT cells and increased with the addition of either LRAP or P172 (a porcine homolog of mouse M180). Thus, we demonstrate the regulation of osteoclastogenesis by LRAP, and the proliferation and migration of cementoblast/periodontal ligament cells by LRAP and P172.

本文言語英語
ページ(範囲)144-149
ページ数6
ジャーナルJournal of Dental Research
85
2
DOI
出版ステータス出版済み - 2 2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • 歯科学(全般)

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