AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle

Masato Miyake, Hideyuki Takahashi, Eri Kitagawa, Hitoshi Watanabe, Takahiro Sakurada, Hisashi Aso, Takahiro Yamaguchi

研究成果: Contribution to journalArticle査読

26 被引用数 (Scopus)

抄録

AMP-activated protein kinase (AMPK) regulates metabolism in skeletal muscle and myostatin (MSTN) negatively regulates skeletal muscle development and growth. In the present study, AMPK activation and the relationship between AMPK and MSTN during myogenic differentiation were investigated in cultures derived from bovine skeletal muscle. Myoblasts capable of forming myotubes were obtained from bovine skeletal muscle and treated with AICAR to activate AMPK, resulting in suppressed myotube formation. AICAR treatment significantly reduced the expression of MSTN mRNA during myogenic differentiation. Combined treatment with AICAR and MSTN suppressed myotube formation to a greater extent than AICAR alone. SB431542, an inhibitor of MSTN signaling, promoted myotube formation during myogenic differentiation. However, simultaneous treatment with AICAR blocked this effect of SB431542. Therefore, AMPK activation inhibits myogenic differentiation but may suppress MSTN expression to balance muscle development.

本文言語英語
ページ(範囲)615-623
ページ数9
ジャーナルCell and tissue research
349
2
DOI
出版ステータス出版済み - 8 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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