An aluminum-resistance mechanism in eucalyptus camaldulensis: Complexation between aluminum and oenothein B in presence of organic acids in vitro

Ko Tahara, Syuntaro Hiradate, Koh Hashida, Kenji Shinohara

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

A hydrolyzable tannin, oenothein B, has recently been identified as a novel type of Al-binding and detoxifying ligand in a highly aluminum (Al)-resistant tree, Eucalyptus camaldulensis. Oenothein B is mainly localized in the root symplast, where the known ligands, citrate and oxalate, are also present. In this study, to investigate whether oenothein B can form complexes with Al in the presence of citrate and oxalate, we analyzed the chemical form of Al in multiple-ligand mixtures containing Al, oenothein B, citrate, and oxalate in vitro at concentrations mimicking those in the symplast of E. camaldulensis roots. In the mixture, 27% of total Al was detected in the insoluble fraction, and 28% in the high-molecular mass (> 10 kDa) soluble fraction. This means that more than half of Al in the mixture interacts with oenothein B, because among the three ligands only oenothein B can predominantly form insoluble or high molecular mass soluble complexes with Al. The27Al-nuclear magnetic resonance analyses of the mixture showed that low-molecular mass soluble Al was not mainly composed of simple Al–citrate or Al–oxalate complexes. These results support our hypothesis that oenothein B contributes to internal detoxification of Al in the symplast of E. camaldulensis roots.

本文言語英語
ページ(範囲)261-264
ページ数4
ジャーナルJournal of Forest Research
22
4
DOI
出版ステータス出版済み - 2017

All Science Journal Classification (ASJC) codes

  • Forestry

フィンガープリント 「An aluminum-resistance mechanism in eucalyptus camaldulensis: Complexation between aluminum and oenothein B in presence of organic acids in vitro」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル