An antimicrobial peptide tachyplesin acts as a secondary secretagogue and amplifies lipopolysaccharide-induced hemocyte exocytosis

Aya Ozaki, Shigeru Ariki, Shun-Ichiro Kawabata

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

In the horseshoe crab, bacterial lipopolysaccharide (LPS) induces exocytosis by granular hemocytes, resulting in the secretion of various defense molecules, such as lectins and antimicrobial peptides, via a G protein-mediating signaling pathway. This response is a key component of the horseshoe crab innate immune response against infectious microorganisms. Here, we report an endogenous amplification mechanism for LPS-induced hemocytes exocytosis. The concentration of LPS required for maximal secretion decreased in proportion to the density of hemocytes, suggesting the presence of a positive feedback mechanism for secretion via a mediator secreted from hemocytes. The exocytosed fluid of hemocytes was found able to induce hemocyte exocytosis in the absence of LPS. Furthermore, tachyplesin, a major antimicrobial peptide of hemocytes, was able to trigger exocytosis in an LPS-independent manner, which was inhibited by a phospholipase C inhibitor, U-73122, and a G protein inhibitor, pertussis toxin. Surface plasmon resonance analysis showed that tachyplesin directly interacts with bovine G protein. These findings suggest that the tachyplesin-induced hemocyte exocytosis also occurs via a G protein-mediating signaling pathway. We concluded that tachyplesin functions not only as an antimicrobial substance, but also as a secondary secretagogue of LPS-induced hemocyte exocytosis, leading to the amplification of the innate immune reaction at sites of injury.

元の言語英語
ページ(範囲)3863-3871
ページ数9
ジャーナルFEBS Journal
272
発行部数15
DOI
出版物ステータス出版済み - 8 1 2005

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Hemocytes
Exocytosis
Lipopolysaccharides
GTP-Binding Proteins
Peptides
Amplification
Horseshoe Crabs
Antigen-antibody reactions
Pertussis Toxin
Surface plasmon resonance
Type C Phospholipases
Lectins
Microorganisms
Tachypleus tridentatus tachyplesin peptide
Surface Plasmon Resonance
Feedback
Innate Immunity
Molecules
Fluids

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

An antimicrobial peptide tachyplesin acts as a secondary secretagogue and amplifies lipopolysaccharide-induced hemocyte exocytosis. / Ozaki, Aya; Ariki, Shigeru; Kawabata, Shun-Ichiro.

:: FEBS Journal, 巻 272, 番号 15, 01.08.2005, p. 3863-3871.

研究成果: ジャーナルへの寄稿記事

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