An approach for formation of vascularized liver tissue by endothelial cell-covered hepatocyte spheroid integration

研究成果: Contribution to journalArticle査読

57 被引用数 (Scopus)

抄録

Tissue vascularization in vitro is necessary for cell transplantation and is a major challenge in tissue engineering. To construct large and regularly vascularized tissue, we focused on the integration of endothelial cell-covered spheroids. Primary rat hepatocytes were cultured on a rotary shaker, and 100-150μm spheroids were obtained by filtration. The hepatocyte spheroids were coated with collagen by conjugation with a type 1 collagen solution. Collagen-coated hepatocyte spheroids were cocultured with human umbilical vein endothelial cells (HUVECs), and monolayered HUVEC-covered hepatocyte spheroids were constructed. Without a collagen coat, many HUVECs invaded hepatocyte spheroids but did not cover the spheroid surface. To construct regularly vascularized tissue, we packed HUVEC-covered hepatocyte spheroids in hollow fibers used for plasma separation. Packed spheroids attached to each other forming a large cellular tissue with regular distribution of HUVECs. At day 9 after packing, HUVECs invaded the hepatocyte spheroids and a dense vascular network was constructed. Collagen coating of spheroids is useful for the formation of endothelial cell-covered spheroids and subsequent regular vascularized tissue construction.

本文言語英語
ページ(範囲)2029-2037
ページ数9
ジャーナルTissue Engineering - Part A
15
8
DOI
出版ステータス出版済み - 8 1 2009

All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生化学
  • 生体材料
  • 生体医工学

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