An asparagine residue regulating conductance through P2X2 receptor/channels

Ken Nakazawa, Kazuhide Inoue, Yasuo Ohno

研究成果: Contribution to journalArticle査読

18 被引用数 (Scopus)

抄録

Single channel currents were recorded from Xenopus oocytes expressing wild-type and mutated P2X2 receptors. When 100 mM Na+ was used as the permeant cation, unitary currents of about 80 pS were recorded from the oocyte expressing the wild-type channels. The single channel conductance was roughly halved when Asn333 was replaced by lie (N333I). A similar decrease in single channel currents was also observed when 100 mM Li+ or Cs+ was used as the permeant cation. With two other mutants, in which Asp315 was replaced by Val (D315V) or Tyr330 was replaced by Ile (T333I), single channel conductance was almost the same as that of the wild-type channels. The results suggest that Asn333, which is believed to be involved in the channel pore, plays an essential role in ion transport through P2X2 receptor/channels.

本文言語英語
ページ(範囲)141-144
ページ数4
ジャーナルEuropean Journal of Pharmacology
347
1
DOI
出版ステータス出版済み - 4 17 1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • 薬理学

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