An ATP gate controls tubulin binding by the tethered head of kinesin-1

Maria C. Alonso, Douglas Robert Drummond, Susan Kain, Julia Hoeng, Linda Amos, Robert A. Cross

研究成果: ジャーナルへの寄稿記事

84 引用 (Scopus)

抄録

Kinesin-1 is a two-headed molecular motor that walks along microtubules, with each step gated by adenosine triphosphate (ATP) binding. Existing models for the gating mechanism propose a role for the microtubule lattice. We show that unpolymerized tubulin binds to kinesin-1, causing tubulin-activated release of adenosine diphosphate (ADP). With no added nucleotide, each kinesin-1 dimer binds one tubulin heterodimer. In adenylyl-imidodiphosphate (AMP-PNP), a nonhydrolyzable ATP analog, each kinesin-1 dimer binds two tubulin heterodimers. The data reveal an ATP gate that operates independently of the microtubule lattice, by ATP-dependent release of a steric or allosteric block on the tubulin binding site of the tethered kinesin-ADP head.

元の言語英語
ページ(範囲)120-123
ページ数4
ジャーナルScience
316
発行部数5821
DOI
出版物ステータス出版済み - 4 6 2007
外部発表Yes

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Kinesin
Tubulin
Adenosine Triphosphate
Microtubules
Adenylyl Imidodiphosphate
Adenosine Diphosphate
Nucleotides
Binding Sites

All Science Journal Classification (ASJC) codes

  • General

これを引用

Alonso, M. C., Drummond, D. R., Kain, S., Hoeng, J., Amos, L., & Cross, R. A. (2007). An ATP gate controls tubulin binding by the tethered head of kinesin-1. Science, 316(5821), 120-123. https://doi.org/10.1126/science.1136985

An ATP gate controls tubulin binding by the tethered head of kinesin-1. / Alonso, Maria C.; Drummond, Douglas Robert; Kain, Susan; Hoeng, Julia; Amos, Linda; Cross, Robert A.

:: Science, 巻 316, 番号 5821, 06.04.2007, p. 120-123.

研究成果: ジャーナルへの寄稿記事

Alonso, MC, Drummond, DR, Kain, S, Hoeng, J, Amos, L & Cross, RA 2007, 'An ATP gate controls tubulin binding by the tethered head of kinesin-1', Science, 巻. 316, 番号 5821, pp. 120-123. https://doi.org/10.1126/science.1136985
Alonso, Maria C. ; Drummond, Douglas Robert ; Kain, Susan ; Hoeng, Julia ; Amos, Linda ; Cross, Robert A. / An ATP gate controls tubulin binding by the tethered head of kinesin-1. :: Science. 2007 ; 巻 316, 番号 5821. pp. 120-123.
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