An attempt to produce transgenic chicken mediating sperm cells as vectors

M. Hasebe, Tomoki Soh, M. Hattori, N. Fujihara

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

An attempt was made to produce possible transgenic chicken via sperm cells as vectors. In vitro incubation of ejaculated chicken spermatozoa with exogenous gene (MiwZ DNA) showed a possibility of the incorporation of foreign gene into sperm cells, which was examined by the PCR analysis. The embryos obtained from the eggs inseminated with DNA-treated spermatozoa indicated an existence of transferred exogenous gene in the blastodermal cells of the incubated fertile eggs. Exogenous gene injected into rooster testes was neither detected in the sperm cells nor in the blastodermal cells of fertilized eggs, which were inseminated with spermatozoa obtained from the testes-injected rooster. Sperm head, tail and cell membrane separated from DNA-treated spermatozoa revealed to contain foreign DNA, suggesting a possibility of incorporation of exogenous gene into plasma membrane from treated-spermatozoa.

本文言語英語
ページ(範囲)143-150
ページ数8
ジャーナルJournal of Applied Animal Research
14
2
DOI
出版ステータス出版済み - 1 1 1998

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

フィンガープリント 「An attempt to produce transgenic chicken mediating sperm cells as vectors」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル